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allow to detect differences between organisms or differences between parts of the same organism
differential stain in which a decolorization step occurs between the application of two basic stains
forms a crystal-violet iodine complex to enhance the crystal violet stain
what does iodine do after stained with crystal violet
gram negative cells
these cells are decolorized by the solution (alcohol or acetone) whereas gram positive are not
gram negatives have higher lipid content because of the outer membrane and a thinner peptidoglycan layer than gram positive cell walls
gram negative cells are able to resist decolorization due to
_________ in the decolorizer extracts the lipid making the gram negative wall more porous and incapable of retaining the crystal violet iodine complex thereby decolorizing it
thick peptidoglycan wall
the reason why gram positive cells are less susceptible to decolorization is because of
older gram positives
may lose their ability to resist decolorization and give an artificial gram - result
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