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Lab exam review from old tests
Terms in this set (46)
HVAC (heating, ventilation, and air conditioning system)
must be reliable; must be able to closely regulate temperature and humidity
How many air changes per hour is the general standard for rooms?
10 - 15
What is recommended to control cross contamination?
air pressure differentials and/or high efficiency particulate air filters (HEPA filters)
positive pressure rooms
air moves out; used in rooms with immune compromised animals e.g. surgery suites
negative pressure rooms
air rushes in when door opens. pressure lower than hallway
What are barrier rooms designed to do?
prevent entry of a disease into a room (bioexclusion)
or prevent disease inside a room from getting out (biocontainment)
Experimental protocols are designated into 4 animal biosafety levels; 1 = lowest risk, 4 = highest risk
rack cage washer
used to sanitize large pieces o equipment
smaller version of rack washer, constructed in configuration of large cabinet
equipment/caging carried through various stations on a conveyor belt; sanitized items exit opposite side of tunnel
What temperature is suggested for achieving sanitation in cage washers?
reduction of the number of microorganisms on object to an acceptable public health standard
a process that destroys pathogenic microorganisms and inhibits their growth
destruction of all organisms on an object
RODAC plate testing
measures bacterial colonies remaining on sanitized equipment
ATP bioluminescent technology
measures presence of adenosine triphosphate, molecule present in all living organisms
What is a microisolation system?
Refinement of the shoe box cage.
allows greater control of the cage environment.
filtered plastic top is placed on top of the plastic bottom, prevents exchange of microorganisms between the cage and environment.
static - without forced ventilation
ventilated - attached to a ventilated system
set on shelf or hung like shoe box systems.
Why are suspended cages discouraged?
It may produce foot problems
Where can you find space recommendations for housing?
Animal Welfare Act
What species is the J-type feeder most often used?
rabbits, ferrets, and chinchillas
used in studies where urine or feces collection is required
manual, crank, mechanical motor.
back of cage moves forward to provide mechanical restraint.
must contain environmental enrichment and perches.
Examples of outbred stocks of mice?
Swiss Webster, CD-1, ICR
What is the purpose of inbred strains of mice?
bred to maintain maximum genetic homogeneity
How many generations of brother/sister or parent/offspring matings are there?
deficient in T-cell lymphocytes
severe combined immunodeficient mice (scid/scid)
deficient in both T-cells and B-cells
first generation of mating between 2 different inbred strains.
DNA orchestrates the function of an organism
abnormal behavior; removal of hair and whiskers from faces, heads, and bodies of other mice
large groups of females housed together, then exposed to male odor
aborting pregnancy; recently bred (4d) female mouse exposed to stange male odor
female mice housed in group without male exposure become synchronized in estrus
with absence of male pheromones, females have suppressed estrus cycle
What is the light requirement for mice?
What is most commonly used for euthanasia in mice?
What are the 3 most common types of mites found in mice?
Myobia musculi, radfordia affinis, mycopotes musculinus
What are 4 special considerations regarding small mammal anesthesia?
1. small size
2. handling is important
3. They don't vomit - fasting not as important
What is important to remember regarding fasting in small mammals?
Except for the ferret, none of these species vomit
Fasting recommendations are not standardized
if performing surgery on the GIT, fast 3-4 hours
What pre-anesthetic agent should not be used in rabbits?
Atropine is broken down quickly in rabbits to to atropinase
What is the safest method for anesthetizing rodents?
How should you anesthetize rabbits if unable to intubate?
maintain with mask
What is used to prevent laryngospasm?
How soon should you offer food and water after recovery in small mammals?
How should you manage respiratory depression in an emergency situation?
Extend head adn neck adn press on chest
put 3cc syringe casing on nose adn blow
Doxapram (respiratory) Epinephrine (cardiac)
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