Study sets, textbooks, questions
Upgrade to remove ads
Hemoglobin and Myoglobin in Medicine
Terms in this set (27)
Stores O2 in the muscle
Red blood cells, transports O2 from lungs to tissues
Monomer, 1 subunit, 1 domain, alpha helices and random coils
Percent of similarity between Hb and Mb, describe structural differences
Extra loop in Mb compared to Hb alpha
Tetramer (2 alpha and 2 X subunits are different)
Non-alpha subunits in adults, fetuses, adult human minor form.
Free heme binds __ very tightly.
This is why we need protein around heme to hinder CO binding.
Oxygen ___ Fe __ to Fe __ in a heme group. This causes...
Iron 3 can't carry oxygen anymore.
Change in color of heme can be detected by ___ or ___.
Eyes or spectrophotometry (shows how oxygenated heme is)
Hb oxygen binding is ___ and we know this because the linear graph has the following shape...
Sigmoidal at beginning, hyperbolic after that.
Myoglobin binding curve shape
Which binds more tightly? Hb or Mb
(Pulls oxygen off of hemoglobin)
Hb cooperativity comes from
Conformational change of Hb tetramer, caused by oxygen binding.
BPG (bisphosphoglycerate) is an ________ regulator of Hb. How many BPG's bind per Hb tetramer? What does binding site look like?
1 molecule binds per tetramer of Hb.
Lots of positively charged side chains in binding site (negative ligand)
BPG binds preferentially to ___ form of Hb. Increasing amount of BPG causes equilibrium shift...
Equilibrium shift toward deoxy form of Hb (helps deliver oxygen to tissues).
Without BPG, Hb binding curve resembles ___.
BPG can be up regulated by ___ and down regulated by ___.
What is a difference between fetal Hb and adult Hb?
Serine side chain in BPG binding site, hydroxyl group which is polar but not enough to stabilize BPG binding.
Other 2 molecules that cause allostery in Hb
H+ (weakens O2 binding)
CO2 (lowers O2 affinity and reversible binding at N-terminal amines)
Hb and Mb moonlighting function
Change NO to NO3- which oxidizes Fe II to Fe III
Hb and BPG formations at lungs
O2 high concentration, Hb forces to high affinity state, BPG forced to dissociate
Hb and BPG formation at fingertips
O2 low, dissociates from hemoglobin, reverts to low-affinity state, BPG binds to facilitate low affinity state.
Methemoglobinemia and antidote
Fe III can't bind oxygen, can never reach high oxygen saturation. Antidote: methylene blue
How does one CO binding to Hb Impact all binding sites?
1 binding locks the rest of the sites in high-oxygen-affinity state (can't carry as much, can't release what we have in the tissues)
Loss of 1 Hb subunit
Don't have proper ratio (stoichiometry). Extra units accumulate and block things, also can't bind oxygen very well.
First one = sickle cell anemia
Mutation of amino acid side chain
Ex: Hb Kansas, Asn to Thr (key location for quaternary conformational change)
Problems identified with synthetic blood attempts
Free Hb cannot withstand the stress/pressures of circulation, free Hb scavenged NO in blood cells
Sets found in the same folder
Integration Case: Alzheimer's Disease
pH, pKa, and the Henderson-Hasselbalch Equation
Other sets by this creator
Overview of Biochemical Reactions and Catalysis
Proteins in Medicine
Determinants of Health: Healthy People 2020
Fluid Mosaic Model
Acidic oxides such as carbon dioxide react with basic oxides like calcium oxide (CaO) and barium oxide (BaO) to form salts (metal carbonates). (a) Write equations representing these two reactions. (b) A student placed a mixture of BaO and CaO of combined mass 4.88 g in a 1.46-L flask containing carbon dioxide gas at 35 degree C and 746 mmHg. After the reactions were complete, she found that the CO2 pressure had dropped to 252 mmHg. Calculate the percent composition by mass of the mixture. Assume volumes of the solids are negligible.
Why is the freezing of water considered to be a physical change?
For a positive charge moving in the direction of the electric field,?
An animal that relies on interaction with the environment to help it control body temperature is known as a(an) a. endotherm. b. ectotherm. c. flightless bird. d. endoderm.
Recommended textbook solutions
Pharmacology and the Nursing Process
Julie S Snyder, Linda Lilley, Shelly Collins
The Human Body in Health and Disease
Gary A. Thibodeau, Kevin T. Patton
Clinical Reasoning Cases in Nursing
Julie S Snyder, Mariann M Harding
Medical Language for Modern Health Care
David M Allan, Rachel Basco
Other Quizlet sets
2020Red Level 6TH Chapter 1 Lesson 3 The…
Health 2600 Exam 1
LG chapter 1