18 terms

Chapter 9

Biotechnology and Recombinant DNA
genetic engineering
artificial gene manipulation
the use of microbiology and biochemistry to make useful products
restriction enzymes
cut DNA at certain sequences
DNA cloning
putting a piece of DNA into a cell and allowing it to replicate
a carrier, usually plasmids or viruses
dissimilation plasmids
have genes for unusual metabolic enzymes
bacteriocinogenic plasmids
have genes for bacteriocins, which are bacterial toxins
resistance plasmids
have genes that help against harmful sustances
conjugative plasmids
have genes that cause transfer b/w 2 microbes
genetically engineered products
horomes, enzymes, vaccines, sturdier plants, snomax
reverse transcriptase
an enzyme that makes a piece of DNA from mRNA
the new DNA that has been made from mRNA
single-stranded pieces of DNA or RNA that have been labelled with a dye or radioactive material
denatured DNA
a DNA molecule that has been split apart
hybridized DNA
pieces of DNA from different sources that have joined together
gel electrophoresis
a substance is soaked into a gel and then electrical current is applied to the gel to cause the substance to move through it
Southern Blot
the gel is placed onto filter paper so the material soaks into it
the process of rapidly copying DNA by heating it until it separates, then using an enzyme from a thermophile to copy the strands over and over