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Lab 1.3: Simulating Osmotic Pressure Lab Report
Terms in this set (13)
Osmosis is a type of what?
what happens when a hypertonic solution is added to cells?
•Directly rt concentration of nondiffusing solutes
•Inversely rt volume
•Directly rt temp/ # of particles in the solution
variables that affects osmotic pressure are
The net movement of water would be into the cell in a?
Sodium is able to diffuse through the pores/ solute concentrations are the same
When does no pressure change occur?
If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
Which membrane did not allow Na+ Cl- to pass through?
equilibrium is reached.
When a solute is able to diffuse through a membrane
Water diffuses towards where?
Increasing the NaCl increased the pressure. When the NaCl concentration increase from 5 to 10, the pressure increased from 170 to 340. The pressure increased because water diffuses to a higher concentration gradient, so the pressure will increase.
Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction?
Osmosis is similar to simple diffusion in the fact that neither methods require any energy. They move molecules (and water) by a concentration gradient from high to low. They differ because in osmosis, only water solvents are transported through the membrane. Simple diffusion moves ions and and molecules across a membrane.
Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Water chases milliosmoles because as the solute concentration increases (the number of milliosmoles rise), the concentration of water will decrease; water moves into areas of high concentration.
Solutes are sometimes measured in milliosmoles. Explain the statement, "Water chases milliosmoles."
There was pressure above the left beaker because the 10mM glucose was small enough to diffuse but the 9mM albumin was not. The albumin in the left beaker built up because it could not diffuse and the pressure increased only on the left side.
The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction?
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