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Chapter 20 Mastering Biology

Terms in this set (30)

Which enzyme(s) will produce a DNA fragment that contains the entire vgp gene (shown in red) and has "sticky ends"?
Select all that apply.
HindIII
EcoRI
HaeIII
HindIII
HindIII
HindIII
Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene?
BamHI, HaeIII, and HindIII
which enzyme(s) would cut the plasmid without disrupting the function of the ampR gene?
BamHI, EcoRI, and HaeIII
Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid?
BamHI, EcoRI, and HindIII
Which one resitriction enzyme satisfies all three of the requirements listed above?
BamHI only
Part C - Transformation and selection of recombinant bacteria
-will grow only in medium without ampicillin: no plasmid
-will grow in both media: recombinant plasmid but no vgp gene, nonrecombinant plasmid, recombinant plasmid with vgp gene
Part D - Screening the plasmid library
TCACACTG...GAATTCAT
What information can not be obtained from the sequence of a gene?
Whether the gene is methylated.
What is the polymerase chain reaction (PCR)?
A method to amplify a fragment of DNA.
True or false? Comparison of the sequences of the same gene across species can give some insight into the existence of a common ancestor with that gene.
True
True or false? The Taq enzyme is a type of DNA polymerase that allows researchers to separate the DNA strands during the annealing step of the PCR cycle without destroying the polymerase.
False
How many DNA molecules would there be after four rounds of PCR if the initial reaction mixture contained two molecules?
32
During which step in the PCR cycle are nucleotides used?
Extension.
During which step in the PCR cycle do primers form bonds with a single-stranded template?
Annealing
The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
sticky ends
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