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5 Written questions

5 Matching questions

  1. Cytochromes
  2. ETC without )₂
  3. Oxidation
  4. Proton Gradient
  5. Metabolic Map
  1. a - most of the molecules of the ETC
    - electron carriers that resemble hemoglobin in structure of their active site
    - functional unit contains a central iron atom, which is capable of undergoing a reversible redox reaction
  2. b ...
  3. c - as NADH passes its electrons to the ETC, free H⁺ are released and accumulate in mitochondrial matrix
    - ETC pumps these ions out of the matrix, across the inner mitochondrial membrane and into intermembrane space at each of the three protein complexes
    - the continuous translocation of H⁺ creates a positively charged acidic environment in the intermembrane space
  4. d - without oxygen, ETC becomes backlogged with electrons and NAD⁺ can't be regenerated to continue glycolysis without lactic acid fermentation occuring
    - Cyanide and dinitrophenol works the same way.
    - Cyanide blocks the transfer of electrons from Cytochrome a₃ to O₂
    - Dinitrophenol uncouples the electron transport chain from the proton gradient established across the inner mitochondrial membrane
  5. e - loss of an electron
    - NAD⁺, FAD, NADP⁺ are referred to as oxidizing agents because they cause other molecules to lose electrons and undergo oxidation (while they're reduced NADH, FADH₂, NADPH)

5 Multiple choice questions

  1. - the body degrades amino acids only when there isn't enough carbs available
    - most amino acids undergo a transamination reaction where they lose an amino group to form an alpha-keto acid
    - carbon atoms of most amino acids are converted into acetyl CoA, pyruvate or one of the intermediates of the citric acid cycle
  2. - occurs in yeast and bacteria only
    - pyruvate produced in glycolysis is decarboxylated to acetaldehyde, then reduced by NADH in step 5 of glycolysis to yield ethanol
    - pyruvate --> acetaldehyde --> ethanol
  3. - the sum total of all chemical reactions that take place in a cell
    - either anabolic (require energy) or catabolic (release energy)
  4. - last carrier of the ETC
    - passes its electron to the final eectron acceptor, O₂
    - in addition, O₂ picks up a pair of hydrogen ions from the surrounding medium and forms water
    - 2H⁺ + 2e⁻ + ½ O₂ --> H₂O
  5. 2 Acetyl CoA + 6 NAD⁺ + 2 FAD + 2 ATP + 2Pi + 4H₂O

    -->

    4 CO₂ + 6 NADH + 2 FADH₂ + 2 ATP + 4 H⁺ + 2 CoA

5 True/False questions

  1. Electron Carriers- categorized into three large protein complexes:
    a) NADH dehydrogenase
    b) the b-c₁ complex
    c) cytochrome oxidase

          

  2. Heterotrophic- obtain energy catabolically
    - break down organic nutrients that must be ingested

          

  3. The Citric Acid Cyle continued- electrons are transferred to NAD⁺ and FAD, generating NADH and FADH₂, which transport electrons to electron transport chain, where ATP is produced via oxidative phosporylation
    - each molecule of glucose = 2 pyruvates
    2x3 NADH --> 6 NADH
    2x1 FADH₂ --> 2 FADH₂
    2x1 GTP (ATP) --> 2 ATP

          

  4. Glycolytic Pathway- fructose 1,6-diphosphate is split into dihydroxyacetone and glyceraldehyde 3-phosphate (PGAL)
    - dihydroxyacetone is isomerized into PGAL
    - two molecules of PGAL is formed per molecule of glucose
    - 1 glucose = 2 pyruvate
    - net production of 2 ATP/mole of glucose (4 generated, 2 used up)

          

  5. ADP- adenosine monophosphate
    - PPi: phyrophosphate
    - ATP --> AMP + PPi + 7 kcal/mole