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5 Written questions

5 Matching questions

  1. Proton Gradient
  2. Cytochromes
  3. NAD⁺
  4. Lactic Acid Fermentation
  5. Carbohydrates
  1. a - as NADH passes its electrons to the ETC, free H⁺ are released and accumulate in mitochondrial matrix
    - ETC pumps these ions out of the matrix, across the inner mitochondrial membrane and into intermembrane space at each of the three protein complexes
    - the continuous translocation of H⁺ creates a positively charged acidic environment in the intermembrane space
  2. b - most of the molecules of the ETC
    - electron carriers that resemble hemoglobin in structure of their active site
    - functional unit contains a central iron atom, which is capable of undergoing a reversible redox reaction
  3. c - occurs in certain fungi and bacteria and in human muscle cells during strenuous activity
    - happens when oxygen supply to muscle cells lags behind the rate of glucose catabolism
    - pyruvate generated is reduced to lactic acid, which can lower blood pH if accumulated, eventually becomes muscle fatigue
    - oxygen debt: the amount of oxygen needed to oxidize lactic acid back to pyruvate and enters cellular respiration
  4. d - disaccharides are hydrolyzed into monosaccharides
    - then converted into glucose or glycolytic intermediates
    - glycogen in the liver can be converted into glucose 6-phosphate, a glycolytic intermediate
  5. e nicotinamide adenine dinuclotide

5 Multiple choice questions

  1. - electrons are transferred to NAD⁺ and FAD, generating NADH and FADH₂, which transport electrons to electron transport chain, where ATP is produced via oxidative phosporylation
    - each molecule of glucose = 2 pyruvates
    2x3 NADH --> 6 NADH
    2x1 FADH₂ --> 2 FADH₂
    2x1 GTP (ATP) --> 2 ATP
  2. 6CO₂ + 2H₂O + energy --> C₆H₁₂O₆ + 6O₂
  3. glucose + 2ADP + 2Pi + 2 NAD⁺


    2 pyruvate + 2ATP + 2NADH + 2H⁺ + 2H₂O
  4. - last carrier of the ETC
    - passes its electron to the final eectron acceptor, O₂
    - in addition, O₂ picks up a pair of hydrogen ions from the surrounding medium and forms water
    - 2H⁺ + 2e⁻ + ½ O₂ --> H₂O
  5. - loss of an electron
    - NAD⁺, FAD, NADP⁺ are referred to as oxidizing agents because they cause other molecules to lose electrons and undergo oxidation (while they're reduced NADH, FADH₂, NADPH)

5 True/False questions

  1. Fats- stored in adipose tissue in the form of triglyceride
    - when needed, they are hydrolyzed by lipases to fatty acids and glycerol, and are carried by the blood to other tissues for oxidation
    - glycerol can be converted into PGAL
    - a fatty acid must be "activated" first in the cytoplasm, this requires 2 ATP
    - on active, it is transorted into mitochondrion and taken through a series of "beta-oxidation cycles" that convert it into two carbon fragments, then converted to acetyl CoA, which enter TCA cycle.
    - each round of beta oxidation generates 1 NADH and 1 FADH₂
    -fats yield the most ATP per gram


  2. ATP- adenosine triphosphate
    - cell's main energy currency
    - synthesized during glucose catabolism
    - composed of nitrogenous base adenine, sugar ribose and three weakly linked phosphate groups
    - energy of ATP is stored in these covalent bonds (high-energy bonds)


  3. Cellular Respiration- most efficient catabolic pathway to harvest energy stored in glucose
    - occurs in mitochondrion and catalyzed by reaction specific enzymes
    - produces 36-38 ATP
    - aerobic, O₂ acts as the final acceptor of electrons that are passed from carrier to carrier during the final stage of glucose oxidation
    - three stages: pyruvate decarboxylation, citric acid cycle and electron transport chain


  4. Fermentation- regeneration NAD⁺ to continue glycolysis without O₂
    - reduce pyruvate to ethanol or lactic acid
    - fermentation produces only 2 ATP per glucose molecule


  5. Pyruvate Decarboxylation- pyruvate formed during glycolysis is transported from the cytoplasm into the mitochondrial matrix where it is carboxylated (lost a CO₂), and the remaining acetyl group is transfered to coenzyme A to form acetyl CoA.
    - in process, NAD⁺ is reduced to NADH
    - pyruvate + coenzyme A -- acetyl CoA