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5 Written questions

5 Matching questions

  1. Fate of Pyruvate
  2. Net reaction of photosynthesis
  3. ATP
  4. Carrier Coenzymes
  5. FMN (flavin mononuclotide)
  1. a - anaerobic: pyruvate is reduced through fermentation
    - aerobic: pyruvate is further oxidized during cell respiration in mitochondria
  2. b 6CO₂ + 2H₂O + energy --> C₆H₁₂O₆ + 6O₂
  3. c - NAD⁺, FAD, NADP⁺
    - transport the high energy electrons of the hydrogen atoms to a series of carrier moelcules on the inner mitochondrial membrane (electron transport chain)
  4. d - first molecule of the ETC
    - reduced when it accepts electrons from NADH, therefore oxidizing NADH to NAD⁺
  5. e - adenosine triphosphate
    - cell's main energy currency
    - synthesized during glucose catabolism
    - composed of nitrogenous base adenine, sugar ribose and three weakly linked phosphate groups
    - energy of ATP is stored in these covalent bonds (high-energy bonds)

5 Multiple choice questions

  1. - when glucose supplies run low, the body uses these (in order): carbohydrates, fats and proteins
    - these are first converted to either glucose or glucose intermediates, which can be degraded in the glycolytic pathway and TCA cycle
  2. - fructose 1,6-diphosphate is split into dihydroxyacetone and glyceraldehyde 3-phosphate (PGAL)
    - dihydroxyacetone is isomerized into PGAL
    - two molecules of PGAL is formed per molecule of glucose
    - 1 glucose = 2 pyruvate
    - net production of 2 ATP/mole of glucose (4 generated, 2 used up)
  3. - there are energy losses as electrons are transferred from one complex to the next, this energy is then used to synthesize 1 ATP per complex
    - since we have 3 complexes, we generate 3 ATP
    - NADH delivers its electrons to NADH dehydrogenase complex, so for each NADH = 3 ATP
    - FADH₂ bypasses the NADH dehydrogenase complex and delivers directly to carrier Q (ubiquinone), which is between complex 1 and 2, so each FADH₂ = 2 ATP
  4. - molecular carriers used by the cell to shuttle energy between reactions
    - ATP, NAD⁺, FAD
  5. nicotinamide adenine dinuclotide

5 True/False questions

  1. AMP- adenosine monophosphate
    - PPi: phyrophosphate
    - ATP --> AMP + PPi + 7 kcal/mole


  2. Cellular Metabolism- most efficient catabolic pathway to harvest energy stored in glucose
    - occurs in mitochondrion and catalyzed by reaction specific enzymes
    - produces 36-38 ATP
    - aerobic, O₂ acts as the final acceptor of electrons that are passed from carrier to carrier during the final stage of glucose oxidation
    - three stages: pyruvate decarboxylation, citric acid cycle and electron transport chain


  3. Proton-Motive Force- from proton gradient
    - drives H+ back across inner membrane and into the matrix
    - membrane is impermeable to ions, so H⁺ must flow through specialized channels provided by enzyme complexes called ATP synthetases
    - as H⁺ pass through ATP synthetases, energy is released to allow for the phosphorylation of ADP to ATP
    - oxidative phosphorylation: coupling of oxidation of NADH with phosphorylation of ADP


  4. Pyruvate Decarboxylation- ATP is produced when high energy potential electrons are transferred from NADH and FADH₂ to oxygen by a series of carrier molecules located in the inner mitochondrial membrane
    - as the electrons are transferred from carrier to carrier, free energy is released
    - later this energy is used to form ATP


  5. Proteins- stored in adipose tissue in the form of triglyceride
    - when needed, they are hydrolyzed by lipases to fatty acids and glycerol, and are carried by the blood to other tissues for oxidation
    - glycerol can be converted into PGAL
    - a fatty acid must be "activated" first in the cytoplasm, this requires 2 ATP
    - on active, it is transorted into mitochondrion and taken through a series of "beta-oxidation cycles" that convert it into two carbon fragments, then converted to acetyl CoA, which enter TCA cycle.
    - each round of beta oxidation generates 1 NADH and 1 FADH₂
    -fats yield the most ATP per gram