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microbiology lab 3
Terms in this set (7)
the bacteria appears colored while the background is bright
they do not stain the bacteria, but the color background. The background looks dark while the bacteria appears clear.
negative staining is specially useful for ?
identification of certain bacteria ( spirochetes ) or structures ( bacterial capsules and endospores ) that are normally difficult to stain
bacteria use in the experiment
1- Rhodospirillum - color of bacterium = gray/white
2- Staphylococus epidermis - color of bacterium = gray/white
why is this technique called negative staining ?
because the bacteria is not stained but the background is the bacteria appears clear/white and the background is black
why does nigrosin not stain the bacteria ?
nigrosin is negatively charge and are repelled by bacteria that have the same negative charge
3 situations where negative staining procedure would be use
1- use to accentuate capsules that surround certain bacteria
2-use to determine if known bacterial infections are either gram (+) or gram (-) bacteria due to the composition of the cell wall
3-to accentuate the endospores of the bacteria
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