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38 terms

9.2 and 9.1 Bio

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DNA replication
the process of making a copy of DNA
What's the point of DNA replication?
To provide genetic information for the next cell.
DNA helicase
an enzyme that unwinds and unzips the DNA (double helix) during DNA replication
Replication fork
a Y shaped point that results when the the 2 strands of DNA (double helix) seperates so that the DNA molecule can b seperated
DNA polymerase
an enzyme that catalyzes the formation of the DNA molecule, it adds nucleotides to exposed nitrogen bases.
When does DNA replication occur?
During the synthesis (S) phase.
STEP 1 of DNA replication
DNA helicases unwind the DNa strands by breaking hydrogen bonds that link complementary nitrogen bases. When seperated proteins attach to each strand to keep them apart.
Are hydrogen bonds weak or stong? Why?
Weak. It makes it easier for the DNA to unattach and break apart, making the DNA relication faster.
STEP 2 of DNA replication
Two new double helix's are formed.
How does step 2 occur?
At the replication forks polymerases move along each strand, adding nucleotides to he nitrogen bases (according to base pairing rules).
STEP 3 of DNA replication
The process in step 2 ccurs until all of DNA has been copied and polymerases are signaled to detach. It produces 2 DNA molecuels composed of 1 new and 1 original strand.(nucleotide sequences are identical)
What checks for errors?
DNA polymerases, they have a "proofreading" role
How does the DNA polymerases check for errors and fix them?
it can only add nucleotides to a growing strand if the previous nucleotide is correctly paired to its complementary base, if there is a mismatched nucleotide, it back tracks and replaces the incorrect pairing with the right one.
Double helix
the spiral-staircase structure characteristic of the DNA molecule
nucleotide
a submit consisting of a sugar, a phosphate, and a nitrogen base
Where is the nucleotide found?
In a nucleic acid chain
deoxyribose
a five-carbon sugar that is a component of DNA nucleotides
Base-pairing rules
it states that cytosine pairs with guanine and adenine pairs with thymine in DNA. Adenine pairs with uracil in RNA
complementary base-pairing
a characteristic of nucleic acids in which the sequenceof bases on one trands is paired with the other sequence of bases on a seperate strand.
purines
nitrogen bases made off two rings of carbon and nitrogen atoms
2 examples of purines
Adenine and Guanine
pyrimidines
nitrogen bases made of a single ring of carbon and nitrogen atoms
2 examples of pyrimidines
Cytosine and Thymine
James watson, and Fancis Crick
pieced together model of DNA structure, They found out that DNA molecules are composed of double helixes and each strand is made up of linked nucleotides
Chargaff's observtions
for each organism studied, the amount of A always equaled T and the amount of G always equaled C, the amount of each base varied between different organisms
X-ray diffraction
Scientists used this to study the structures of molecules
The way x-ray diffraction works
x-rays bounce off the object and are scattered in a patteren onto a piece of film
How can scientists determine structure of the molecule?
By analyzing the patterns
Maurice Wilkins an Rosalind Frankiln
developed high-quality X-ray diffraction pictures of DNA strands, suggestng that it resembled a tightly coiled helix and was composed of two or three strands of nuleotides
Wton an crick determined...
that purine on one strand of DNA always goes with a pyrimidine on the opposite strand
Pairing between bases
adenine forms 2 hydrogen bonds with thymine, cytosine forms 3 hydrogen bonds with guanine
plasmid
circular DNA found in bacteria
(true or false) Repliation begins at one end of DNA molecule and ends at the other end
False
Prokaryote DNA repliaton
2 replicated forks that begin at a single point and the replicating forks move away from eachother until they meet the opposite side of the DNA circle
Eukaryotic cells
each chromosome contains a single long strand of DNA
length of DNA makes it a challenge
the replication of a typiclal human chromosome with one pair of replication forks spreading rom a single point takes 33 days
Human chromosome replication
each chromo is replicated in 100 sections that are 100000 nucleotides long with a different starting point
An entire human chromome can be replicated in...
8 hours