Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Salmonella sp. from similar organisms?
VP positive Urease positive Lysine positive
What is the break down product of Lysine?
What is the break down product of Ornithine and Arginine?
What is the color of a positive Nitrate, Indole, Urease, and VP test?
You are performing a biochemical test on an isolated colony of bacteria. You perform an oxidase test, what color would a positive reaction be?
You perform an ONPG test and the reaction color is yellow. Is your result positve or negative?
If you perform a phenylalanine deaminase test and your reaction yields a green color, what is your interpretation of the reaction?
You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase negative, LDC (Lysine decarboxylation) negative. Which bacteria is most likely present?
You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase positive, and Indole positive. Which bacteria is most likely present?
You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely organism?
You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole positive, and Citrate negative. What is the most likely organism?
You have a K/A, H2S producing TSI reaction with biochemical reactions of Phe deaminase negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely identification of the organism?
Which gram negative non fermenting bacteria are associated with cystic fibrosis patients?
Burkholderia cepacia and Pseudmonas aeruginosa
Haemophilus influenzae requires what two growth factors?
X (hemin) and V (NAD)
What test is used to determine sensititvity to ampiciliin when dealing with Haemophilus influenzae?
You receive a sputim sample from a patient who has recently returned from a vacation at a lake. The organism grows on BCYE (Buffered charcoal yeast agar). Which bacteria is present?
What organism is associated with Pseudomembranous Colitis (PMC)?
Which gram positive anaerobe is responsible for causing "Lumpy jaw"?
Which gram positive anaerobe is associated with sulphur granules?
What is the cause and vector responsible for Lyme disease?
Borrelia burgdorferi/Ixodes (deer/mouse) tick
What is the proper procedure to culture and isolate Borrelia burgdorferi?
Culture in Kelly medium at 33C - examining in darkfield microscopy weekly for one month
Which obligate intracellular parasite is identified by a Giemsa stain and growth in yolk sac of chick embryo or tissue culture (McCoy cell)?
How many Mycobacteria must be present on a smear to be considered significant?
Any number >20 >50
In order for a sputum specimen to be considered acceptable when processing for Mycobacteria, what type of collection must it be?
First morning, 3 consecutive mornings
If a specimen suspected of Mycobacteria contamination was received in a non sterile container, would this be criteria for rejection?
Must a specimen for Mycobacteria isolation be collected sterily?
No (the CONTAINER must be sterile)
What chemical is used to decontaminate a Mycobacteria specimen?
Which species of Mycobacteria are photochromogen pigment producers?
Mycobacterium marinum and mycobacterium kansasii
Which species of Mycobacterium are scotochoromogens?
Mycobacterium scrofulaceum and mycobacterium gordonae
Which species of Mycobacterium are non photochromogens?
Mycobacterium avium and mycobacterium ulcerans
If gram positive cocci are identified from a sputum sample, what are the most likely type(s) of bacteria?
Staphylococcus aureus and streptococcus pneumoniae
What bacteria would most likely be associated with peptic ulcers?
What colony count indicates a UTI?
How does transport media differ from that of bacteria media?
Contains nutrients (fetal calf serum or albumin) and antibiotics
Describe the DNA probe method.
Molecular cloning of a specific DNA sequence
Describe the PCR method.
Method in which nucleic acid sequences are amplified in vitro. (Each cycle produces double the amount of desired nucleic products)
Which RNA virus causes Yellow Fever (aka Dengue fever, St. Louis encephalitis)?
Which RNA virus causes Pulmonary syndrome/Hemorrhagic fever?
Which RNA virus causes Measles?
Which RNA virus causes Polio myelitis/Aseptic meningitis?
Which RNA virus causes Rabies?
Which RNA virus causes Acute Infectious Infantile Diarreha?
Which RNA virus causes Rubella?
Which human T-cell leukemia virus is responsible for causing Hairy Cell leukemia?
Which virus is associated with Negri bodies in brain tissue of infected animals?