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82 terms

ASCP Review: Microbiology

Based on "Clinical Laboratory Science Review: A Bottom Line Approach" 4th Ed.
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Which of the following is the correct temperature at which an autoclave must be run?

-37*C
-121*C
-25*C
121*C
How long is an autoclave run?
15 mins
How many lbs per square inch of pressure is necessary when using an autoclave?
15 lbs/sq in
Which of the following is the correct depth and pH of a Mueller Hinton plate?

-6mm and pH 7.2-7.4
-3mm and pH 6.5-6.8
-4mm and 7.2-7.4
4mm and pH 7.2-7.4
What is the McFarland standard for use in the Kirby Bauer method?
0.5 (10^8 organisms)
Which combination of organisms are used as QC for susceptibility testing using the Kirby Bauer method?

-S. pyogenes, P. mirabilis, N. lactamica
-P. vulgaris, S.pyogenes
-E. coli, S.aureus, P. aeruginosa
E. coli, S.aureus, P. aeruginosa
Which anaerobic bacteria is isolated on Bacteroides Bile Esculin Agar (BBE)? What color do the colonies appear as?

-B. fragilis, black colonies
-C. difficile, green colonies
-B. pertussis, yellow colonies
B. fragilis, black colonies
Kanamycin-Vancomycin Laked Blood is used to isolated which anaerobic bacteria?

-Clostridium species
-Legionella species
-Bacteroides species
Bacteroides species
Cycloserine-Cefoxitin Fructose Agar (CCFA) is used to isolate which anaerobic bacteria?

-Mycobacteria species
-C. diptheriae
-C. difficile
C. difficile
What factors is CDC Anaerobic Blood Agar enriched with?

-hemin, cysteine, and vitamin K
-X, V, and hemin
-hemoglobin, X, and V
Hemin, cysteine, and vitamin K
Cooked and or chopped meat is used to aid in the isolation of what type of bacteria?
anaerobes
Bordet-gengou agar isolates which of the following?

-B. fragilis
-B. pertussis
-F. tularensis
B. pertussis
Which media is used to isolate Legionella species?
Buffered Charcoal Yeast Extract
Which media is used to isolate F. tularensis?
Cystine-Glucose Agar
Skirrow agar isolates what species of bacteria?
Helicobacter pylori
Fletcher's medium isolates what bacteria?
Leptospira
Thiosulfate Citrate-Bile Salts (TCBS) is used to isolated which bacteria?
Vibrio species
Vaginalis Agar (human blood) is used to isolated which bacteria?
Gardnerella vaginalis
Both Cystine-Tellurite Blood and Loeffler's medium can be used to isolate which of the following species of Clostridium?

-difficile
-diptheriae
-tetani
C. diptheriae (black colonies)
What is the correct blood to broth dilution when perfoming a blood culture?

- 1:5
- 1:20
- 1:10
1:10
When is the best time to draw a blood culture?

- during a fever spike
- just prior to a fever spike
- just after a fever spike
just prior to a fever spike
What must be used to prep the skin prior to drawing a blood culture?
70% alcohol and iodine
What is the max # of blood culures that may be drawn within a 24 hour period?
3
What is the drug of choice for MRSA?
Vancomycin
S. aureus is most resistant to which antiboitic?
penicillin
Which gram postive cocci appears on a plate as mucoid "water drop" colonies, is sensitive to optochin and is bile soluble?
S. pneumoniae
Which of the following would be the correct identifying biochemical test reaction which would differentiate Citrobacter sp. from similar organisms?

VP positive
Lysine negative
Motility negative
Lysine negative
What would be the correct identifying biochemical test reaction(s) which would differentiate Enterobacter sp. from similar organisms?
VP positive, DNAase negative, Ornithine positive
Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Escherichia sp. from similar organisms?

VP negative
Urease positive
Lysine negative
VP negative
Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Morganella sp., Proteus sp., and Providencia sp. from similar organisms?

Lysine positive
Deaminase positive
Motility negative
Deaminase positive
Which of the following would be the correct identifying biochemical test reaction(s) which would differentiate Salmonella sp. from similar organisms?

VP positive
Urease positive
Lysine positive
Lysine positive
What is the break down product of Lysine?
Cadaverine
What is the break down product of Ornithine and Arginine?
Putrescine
What is the color of a positive Nitrate, Indole, Urease, and VP test?
Pink
You are performing a biochemical test on an isolated colony of bacteria. You perform an oxidase test, what color would a positive reaction be?
Purple
You perform an ONPG test and the reaction color is yellow. Is your result positve or negative?
Positive
If you perform a phenylalanine deaminase test and your reaction yields a green color, what is your interpretation of the reaction?
Positive
You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase negative, LDC (Lysine decarboxylation) negative. Which bacteria is most likely present?
Citrobacter sp.
You have an organism that has an A/A, gas producing TSI reaction and is H2S positive, Phe deaminase positive, and Indole positive. Which bacteria is most likely present?
Proteus vulgaris
You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely organism?
Klebsiella sp.
You have an organism that has an A/A, gas producing TSI reaction and is H2S negative, Indole positive, and Citrate negative. What is the most likely organism?
E. coli
You have a K/A, H2S producing TSI reaction with biochemical reactions of Phe deaminase negative, Indole negative, LDC (Lysine decarboxylase) positive, and ODC (Oxalate decarboxylase) negative. What is the most likely identification of the organism?
Salmonella typhi
Which gram negative non fermenting bacteria are associated with cystic fibrosis patients?
Burkholderia cepacia and Pseudmonas aeruginosa
Haemophilus influenzae requires what two growth factors?
X (hemin) and V (NAD)
What test is used to determine sensititvity to ampiciliin when dealing with Haemophilus influenzae?
Beta lactamase
You receive a sputim sample from a patient who has recently returned from a vacation at a lake. The organism grows on BCYE (Buffered charcoal yeast agar). Which bacteria is present?
Legionella sp.
What organism is associated with Pseudomembranous Colitis (PMC)?
Clostridium dificile
Which gram positive anaerobe is responsible for causing "Lumpy jaw"?
Actinomyces israeli
Which gram positive anaerobe is associated with sulphur granules?
Actinomyces israeli
What is the cause and vector responsible for Lyme disease?
Borrelia burgdorferi/Ixodes (deer/mouse) tick
What is the proper procedure to culture and isolate Borrelia burgdorferi?
Culture in Kelly medium at 33C - examining in darkfield microscopy weekly for one month
Which obligate intracellular parasite is identified by a Giemsa stain and growth in yolk sac of chick embryo or tissue culture (McCoy cell)?
Chlamydia sp.
How many Mycobacteria must be present on a smear to be considered significant?

Any number
>20
>50
Any number
In order for a sputum specimen to be considered acceptable when processing for Mycobacteria, what type of collection must it be?
First morning, 3 consecutive mornings
If a specimen suspected of Mycobacteria contamination was received in a non sterile container, would this be criteria for rejection?
Yes
Must a specimen for Mycobacteria isolation be collected sterily?
No (the CONTAINER must be sterile)
What chemical is used to decontaminate a Mycobacteria specimen?
NaOH
Which species of Mycobacteria are photochromogen pigment producers?
Mycobacterium marinum and mycobacterium kansasii
Which species of Mycobacterium are scotochoromogens?
Mycobacterium scrofulaceum and mycobacterium gordonae
Which species of Mycobacterium are non photochromogens?
Mycobacterium avium and mycobacterium ulcerans
If gram positive cocci are identified from a sputum sample, what are the most likely type(s) of bacteria?
Staphylococcus aureus and streptococcus pneumoniae
What bacteria would most likely be associated with peptic ulcers?
Heliobacter pylori
What colony count indicates a UTI?
>100,000 organisms/mL
How does transport media differ from that of bacteria media?
Contains nutrients (fetal calf serum or albumin) and antibiotics
Describe the DNA probe method.
Molecular cloning of a specific DNA sequence
Describe the PCR method.
Method in which nucleic acid sequences are amplified in vitro. (Each cycle produces double the amount of desired nucleic products)
Which RNA virus causes Yellow Fever (aka Dengue fever, St. Louis encephalitis)?
Flavivirus
Which RNA virus causes Pulmonary syndrome/Hemorrhagic fever?
Hantavirus
Which RNA virus causes Measles?
Morbilivirus
Which RNA virus causes Polio myelitis/Aseptic meningitis?
Poliovirus
Which RNA virus causes Rabies?
Rhabdovirus
Which RNA virus causes Acute Infectious Infantile Diarreha?
Rotavirus
Which RNA virus causes Rubella?
Rubivirus
Which human T-cell leukemia virus is responsible for causing Hairy Cell leukemia?
HTLV II
Which virus is associated with Negri bodies in brain tissue of infected animals?
Rhabdovirus
Which DNA virus causes respiratory infections?
Adenovirus
Which DNA virus causes mental retardation?
Cytomegalovirus
Which DNA virus causes infectious mononucleosis?
Epstein Barr virus
Which DNA virus causes oral/genital infections?
Herpes simplex virus I and II
Which DNA virus causes a cervical carcinoma?
Human Papillomavirus (HPV)
Which DNA virus causes smallpox?
Poxvirus
Which DNA virus causes chickenpox/shingles?
Varicella-zoster virus