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Terms in this set (13)
an assembly of cholesterol, sphingolipids, phospholipids and proteins that exist in phase separation from the bulk of the phospholipid bilayer
omega shaped invaginations of the plasma membrane that affect the tension of the bilayer surface
Laurdan Fluorescence imaging
tail in the membrane and naphthalene ring remains out. As the head group moves the chain reorients according to how the membrane in packed. This happens from a quick response to electronic shift
Fluorescence recovery after photobleaching
take a very defined area and bleach with a laser. This removes your chromophore. You then get a redistribution which tells you how long it takes for the chromophore to migrate back to the area that was bleached
Forster resonance energy transfer
non radiative energy transfer between two moleclar entities separated by distances considerably exceeding the sum of their van der waals radii. If proteins randomly distributed in membrane FRET increases over time or surface density. If proteins in raft then FRET is independent of surface density and time.
Single particle tracking
attach gold particle to antigen fragment using optical tweezers. You can then follow the gold particle with the laser and see where it goes
Compartmentalized fluid model
molecules undergo short term confined diffusion within a compartment of rather homogenous phase, and the "hop" to an opposed "compartment" and diffuse at a particular rate allowed in the new compartment.
voltage applied across membrane which opens a pore that acts as a conductive pathway through the bilayer
Which curvature facilitates fusion?
- expand cis leaflets with positive curvature
- compact trans leaflets with negative curvature
Kiss and Run
used in exocytosis. Proteins bring membranes close and a hemifusion stalk forms, leading to pore formation which is sufficient for exocytosis
proteins embedded in lipid raft and restricted lipid fluidity in raft can cause attachment at either end, resulting in fusion
One membrane is mechanically inserted into another, drawing them together (viral particles)
Amphiphilic Protein Membrane Distribution
Peripheral proteins have one hydrophilic and one hydrophobic side. These proteins wiglle and jiggle and if 2 membranes are close enough the proteins disrupt both membranes which restructure around the protein to form a stalk and the fusion can then occur
THIS SET IS OFTEN IN FOLDERS WITH...
Lipids Midterm 2
Lipids Midterm 1
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