Fuel molecules are ___ compounds that are capable of being ___; of losing electrons.
oxidation-reduction, oxidation, CO2
The citric acid cycle includes a series of ___ reactions that ultimately result in the ___ of the acetyl group to two molecules of ___.
high-transfer-potential, high-energy, ATP
Citric acid cycle oxidations generate ___ or ___ electrons that will be used to power the synthesis of ___.
The function of the citric acid cycle is the ___ of high-energy electrons from ___ fuels.
The cycle consists of two parts: one ___ carbon atoms to CO2 and the other regenerates ___.
oxidizes, CO2, ATP, NADH, FADH2
The citric acid cycle ___ two-carbon units, producing two molecules of ___, one molecule of ___, and high-energy electrons in the form of ___ and ___.
acetyl, four, oxaloacetate, tricarboxylic
In the citric acid cycle, a two-carbon ___ unit condenses with a ___-carbon component of the citric acid cycle, ___, to yield a six-carbon ___ acid.
The six-carbon compound releases CO2 twice, which yields high-energy electrons. A ___-carbon compound remains. This compound is further oxidized to regenerate ___, which can initiate another round fo the cycle.
Two carbon atoms enter the cycle as an ___ unit and two carbon atom leave the cycle in the form of two molecules of ___.
Note that the citric acid cycle neither generates a large amount of ___ nor includes ___ as a reactant.
removes, citrate, NADH, FADH2, nine, oxidative phosphorylation
The citric acid cycle ___ electrons from ___ and uses these electrons to form ___ and ___. These electron carriers yield ___ molecules of ATP when they are oxidized by O2 in ___.
NADH, FADH2, electron-transport, proton, ADP, inorganic phosphate
Electrons released in the reoxidation of ___ and ___ flow through a series of membrane proteins, known as the ___ chain, to generate a ___ gradient across the membrane. This gradient is used to generate ATP from ___ and ___.
oxaloacetate, citrate, CO2, citrate, four
In the first stage of the citric acid cycle, two carbon atoms are introduced to the cycle by coupling with ___ to form ___, and two carbon atoms are released as ___ as ___ is metabolized to a ___-carbon molecule.
In the second stage of the citric acid cycle, the resulting ___-carbon molecule is metabolized to regenerate ___, allowing the continued functioning of the cycle.
cellular, removal, O2, ATP
The citric acid cycle constitutes the first stage in ___ respiration, the ___ of high-energy electrons from carbon fuels. These electrons reduce ___ to generate a proton gradient, which is used to synthesize ___, which constitues oxidative phosphorylation.
oxaloacetate, acetyl, tricarboxylic
In the first part of the citric acid cycle, the four-carbon molecule ___ condenses with a two-carbon ___ unit to yield citrate, a six-carbon ___ acid.
CO2, oxidative decarboxylation, four, two
As citrate moves through the first part of the cycle, two carbon atoms are lost as ___ in a process called ___, which yields a ___-carbon molecule and high-transfer-potential electrons captured as ___ molecules of NADH.
condensation, H2O, citrate
The citric acid cycle begins with the ___ of a four-carbon unit, oxaloacetate, and a two-carbon unit, the acetyl group of acetyl CoA. Oxaloacetate reacts with acetyl CoA and ___ to yield ___ and CoA.
citryl CoA, thioester
Oxaloacetate first condenses with acetyl CoA to from ___, a molecule that is energy rich because it contains a ___ bond that originated in acetyl CoA.
hydrolysis, thioester, two
The ___ of the citryl CoA ___ linkage to citrate and CoA drives the overall reaction far in the direction of the synthesis of citrate, powering the synthesis of a new molecule from ___ precursors.
dimer, active site, adjacent, conformational
Mammalian citrate synthase is a ___ of identical 49-kd subunits. Each ___ is located in a cleft between the large and small domains of a subunit, ___ to the subunit interface. X-ray crystallographic studies revealed that the enzyme undergoes large ___ changes in the course of catalysis.
oxaloacetate, acetyl CoA, oxaloacetate, open, closed, acetyl CoA
The reason for the ordered binding is that ___ induces a major structural rearrangement leading to the creation of a binding site for ___. The binding of ___ converts the ___ form of the enzyme to a ___ form. These structural changes create a binding site for ___.
condensation, citryl CoA, enclosed, citryl CoA, hydrolysis, open
Citrate synthase first catalyzes the ___ of citrate and acetyl CoA to form ___, which induces additional structural changes in the enzyme, causing the active site to become completely ___. The enzyme then cleaves the ___ thioester by ___. CoA leaves the enzyme, followed by citrate, and the enzyme returns to the initial ___ conformation.
hydrolysis, citrate synthase, oxaloacetate, thioester, Induced
The wasteful ___ of acetyl CoA is prevented because ___ is well suited to the hydrolysis of citryl CoA but not acetyl CoA. First, acetyl CoA does not bind to the enzyme until ___ is bound and ready for condensation. Second, the catalytic residues crucial for the hydrolysis of the ___ linkage are not appropriately positioned until citryl CoA is formed. ___ fit prevents an undesirable side reaction.
A major purpose of the citric acid cycle is the ___ of carbon atoms leading to the capture of ___ electrons.
citrate, hydroxyl, decarboxylations, citrate, isocitrate, decarboxylation
The newly formed ___ molecule is not optimally structured for the required oxidation reactions. In particular, the ___ group is not properly located in the molecule for the oxidative ___ that follow. Thus, ___ is isomerized into ___ to enable the six-carbon unit to undergo oxidative ___.
dehydration, hydration, H, OH, aconitase, cis-aconitate
The isomerization of citrate is accomplished by a ___ step followed by a ___ step. The result is an interchange of an ___ and an ___. The enzyme catalyzing both steps is called ___ because ___ is an intermediate.
isocitrate dehydrogenase, oxalosuccinate, α-ketoacid, CO2, NADH
The oxidative decarboxylation of isocitrate to α-ketoglutarate is catalyzed by ___. The intermediate in this reaction is ___, an unstable ___. While bound to the enzyme, it loses ___ to form α-ketoglutarate. This oxidation generates the first high-transfer-potential electron carrier in the cycle, ___.
CO2, five, four, α-ketoglutarate dehydrogenase complex, three, α-ketoacid
Removing ___ from the α-ketoglutarate, ___ carbon atoms, to form succinyl CoA, ___ carbon atoms. This reaction is catalyzed by the ___, an organized assembly of ___ kinds of enzymes that is structurally similar to the PDH complex. In fact, the oxidative decarboxylation of α-ketoglutarate closely resembles that of pyruvate, also an ___.