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Misc. Infectious Disease
Terms in this set (32)
Which disease is caused by Borrelia burgdorferi - spirochete, transmitted through
bites of Ixodes tick carried by white-tailed deer with stages of disease being (1) Localized rash, (2) dissemination to multiple organs such as skin, nervous system, heart and joints and (3) chronic disseminated stage with symptoms of arthritis?
Which disease involves IgM response occurs 3 - 6 weeks after bite and the presence of B. burgdorferi confirmed by direct (culture) or indirect methods (serology)?
What are the laboratory testing involved in diagnosing lyme disease?
Indirect immunofluorescence, Enzyme immunoassay, Immunoblotting (western blot) and PCR.
Which technique is Used to screen with the antigen on slide (spirochete), titers of 256 is considered positive, false negatives may occur in specimens collected early in infection, false positives are caused by syphilis, rheumatoid arthritis, etc. and positives are confirmed by Western Blot?
IFA for lyme's disease
Which techniques is Used to screen, it is more precise than IFA, it lacks sensitivity in early stages of disease causing false negatives, false positives due to syphilis, relapsing fever, leptospirosis, infectious mononucleosis, Rocky Mountain spotted fever and can run RPR to rule out syphilis?
ELISA for Lyme's disease
Which technique is recommended confirmatory test by Electrophoresis of B. burgdorferi antigens in acrylamide gel, transfer of antigens to nitrocellulose paper, paper is reacted with patient serum, antihuman globulin with enzyme tag added, washed and enzyme substrate added then protein bands are numbered according to molecular weight?
Western Blot for Lyme's disease
What is the interpretation of western blot for lyme's disease?
IgM antibody is positive if 2 of the following bands are positive at 23, 39, 42 kD. IgG antibody is positive if 5 of the following bands are positive
18, 23, 28, 30, 39, 41, 45, 58, 66, 93 kD.
Which technique has the best sensitivity and specificity, DNA from patient (skin from rash area) is extracted and amplified
DNA probe (target DNA found only in strains of B. burgdorferi) is added, if positive, the single stranded DNA probe will bind to the exact complimentary strand of patient DNA, no cross reactivity with other spirochete antigens?
PCR for Lyme's disease
What is the causative agent for toxplasmosis?
Toxoplasma gondii an protozoan parasite.
What infectious disease is transmitted through ingestion of contaminated soil, cat litter or infected raw or partially cooked pork, mutton, or beef, blood transfusion, organ transplant and congenitally?
What is possible of congenitally transmitted toxoplasmosis?
Death may occur if exposed in 1st trimester. Later infection may cause blindness, hydrocephaly, or other CNS damage. It isn't transmitted if mother is exposed prior to pregnancy.
Which infectious diseases consists of nearly 40% of the world's population being infected, usually asymptomatic or mild lymphadenopathy and can be sever in immunocompromised?
What is the method of infection of toxoplasmosis and how does it affect phagocytosis?
The organism replicates in macrophages and can survive indefinitely due to its ability to prevent fusion of lysosomes with phagosomes and stop phagocytosis.
What are the serological testing for toxoplasmosis?
Isolation of the organism, EIA for IgM or IgG, IFA for IgG, specific IgM (detectable) and IgA testing of newborns and PCR for prenatal testing on amniotic fluid.
What is the method of choice when performing serological testing for toxoplasmosis?
EIA for IgM or IgG.
Which infectious disease causes infectious gastroenteritis, occurs in infants and small children usually in winter or spring and is transmitted through oral-fecal route?
What are the serological testing done for rotavirus?
Direct EIA and Indirect EIA.
Which technique test for the viral antigen of rotavirus?
Which technique test for the antibody to the viral antigen of rotavirus?
Which infections disease is caused by a ssRNA virus, has respiratory route of entry with symptoms including bilateral or unilateral parotitis (infection of salivary glands: 30-40% of cases) and can spread to meninges of brain, salivary glands, pancreas, testes, and ovaries causing inflammation?
Which infectious disease has an incubation period of 12 to 25 days and have a live attenuated vaccine available?
What is the issue with isolating Toxoplama gondii?
It is difficult and impractical.
What are the laboratory testing for mumps that are important in diagnosing cases without parotitis?
Viral culture, serological testing and RT-PCR.
What is the issue with performing a viral culture in laboratory testing for mumps?
It is slow and difficult.
Serological testing including ELISA and IFA are considered the method of choice. What are some other serological test that can be performed for mumps?
complement fixation, hemagglutination inhibition, neutralization assays.
Which commonly used serological test involve solid-phase mumps specific IgM capture assay reducing false positives dure to RF and specific mumps IgM requiring serial testing for diagnosis of new case?
ELISA and IFA.
Which technique is not widely used at this time for mumps but is available?
Which infectious disease is caused by the rubeola virus (ssRNA) with an incubation period of 10 to 12 days having symptoms to include fever, cough, runny nose, conjunctivitis and eythematous macropapular rash appearing 14 days after exposure and have a vaccine that is usually administered in combo with MMR?
What are the laboratory testing used for measles?
Viral culture and serological testing.
What serological testing are involve in testing for measles?
ELISA, Hemagglutination inhibition, neutralization, complement fixation, IFA and RT-PCR.
Which serological testing consist of Rubeola specific IgM (IgM capture assay reducing false positive due to rheumatoid factor) and IgG and is most commonly used?
What is the issue with viral cultures in laboratory testing for measles?
It is slow and difficult.
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