28 terms

MCAT Lab Techs


Terms in this set (...)

finds presence of antigens (proteins) or specfic immunoglobulins (antibodies)
Separation by size or by charge
Southern blotting
detect presence of specific sequences within heterogenous sample of DNA; isolate and purify target DNA sequences
Northern blotting
determines whether specific gene products are being expressed (if mRNA is present in a cell, they are probs being translated to a protein)
Western blotting
detect presence of certain proteins within a sample
Eastern blotting
analyze post-translational modification of peptides such as addition of lipids or carbohydrates
Thin Layer chromatography
nonpolar mobile phase and polar stationary phase; separate based on polarity; Rf = migration distance/migration distance; Polar compounds are LOWW and SLOWW
Column Chromatography
separation based on polarity; separates large amounts of solids or high BP liquids; polar compounds elute last
Gas Chromatography
separation based on boiling point; used to separate small amounts; compound with lowest BP exit first
Size Exclusion Chromatography
Based on molecular size; Large molecules go around beads and elute first; small molecules elute last
ion exchange
separate based on difference in charge states; stationary phase has charged resin; Flush out what's retained with cations; cation-exchange resin - retains cations;
Immunoaffinity chromatography
separates based on highly sepcific lock and key interactions
metal ion affinity chromatography
uses recombinant proteins (proteins with a tag at its C or N terminus); metal binds a lot of proteins...change pH or add competitive metal ion ligands
based on boiling point; used to separate large amounts of low boiling point compounds
Fractional distillation
BP differences <30 degrees; useful to separate diastereomers
Solvent extraction
separates based on solubility in polar and nonpolar solvents; strong base = phenols and carboxylic acids; weak based deprotonates carboxylic acids; acid deprotonates AMINES
separate enantiomers or diastereomers
Mass spec
used to determine the molecular weight of a compound
indicates what functional groups are present in a molecule
3300 cm
1700 cm
1650 cm
C triple bonded to N /C
2100-2260 cm
looks at connectivity of H atoms; # of signals = # of non-equivalent Hs; splitting pattern: n = # of H's on adjacent carbons (n+1 = # of peaks for a signal)
HNMR - alcohols
HNMR - aldehyde
HNMR - alkyl
HNMR - H - C - N/O/Halogen