28 terms

MCAT Lab Techs

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ELISA
finds presence of antigens (proteins) or specfic immunoglobulins (antibodies)
electrophoresis
Separation by size or by charge
Southern blotting
detect presence of specific sequences within heterogenous sample of DNA; isolate and purify target DNA sequences
Northern blotting
determines whether specific gene products are being expressed (if mRNA is present in a cell, they are probs being translated to a protein)
Western blotting
detect presence of certain proteins within a sample
Eastern blotting
analyze post-translational modification of peptides such as addition of lipids or carbohydrates
Thin Layer chromatography
nonpolar mobile phase and polar stationary phase; separate based on polarity; Rf = migration distance/migration distance; Polar compounds are LOWW and SLOWW
Column Chromatography
separation based on polarity; separates large amounts of solids or high BP liquids; polar compounds elute last
Gas Chromatography
separation based on boiling point; used to separate small amounts; compound with lowest BP exit first
Size Exclusion Chromatography
Based on molecular size; Large molecules go around beads and elute first; small molecules elute last
ion exchange
separate based on difference in charge states; stationary phase has charged resin; Flush out what's retained with cations; cation-exchange resin - retains cations;
Immunoaffinity chromatography
separates based on highly sepcific lock and key interactions
metal ion affinity chromatography
uses recombinant proteins (proteins with a tag at its C or N terminus); metal binds a lot of proteins...change pH or add competitive metal ion ligands
Distillation
based on boiling point; used to separate large amounts of low boiling point compounds
Fractional distillation
BP differences <30 degrees; useful to separate diastereomers
Solvent extraction
separates based on solubility in polar and nonpolar solvents; strong base = phenols and carboxylic acids; weak based deprotonates carboxylic acids; acid deprotonates AMINES
Resolution
separate enantiomers or diastereomers
Mass spec
used to determine the molecular weight of a compound
IR
indicates what functional groups are present in a molecule
OH
3300 cm
C=O
1700 cm
C=C
1650 cm
C triple bonded to N /C
2100-2260 cm
HNMR
looks at connectivity of H atoms; # of signals = # of non-equivalent Hs; splitting pattern: n = # of H's on adjacent carbons (n+1 = # of peaks for a signal)
HNMR - alcohols
2-5
HNMR - aldehyde
10
HNMR - alkyl
0-2
HNMR - H - C - N/O/Halogen
2-4