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Microbiology Ch. 6 Part 1
Terms in this set (36)
What is the greatest cultured microoorganism?
to cultivate or culture microorganisms, a sample called an ____ is introduced into a collection of nutrients called a _______
Microogranisms that grow from an inoculum; can refer to the act of cultivating microorganisms or to the microorganisms that are cultivated
cultures are grown in what type of media?
liquid (broths) or surface of solid media
What are the 5 "I"s of culturing microbes?
cultures that are visible on the surface of solid media
introduction of a sample into a container of media to produce a culture of observable growth
separating one species from another to look at microbes individually or to work in pure culture
placing a sample under conditions that allow growth
incubation- you will most often regulate?
sample of human material, such as feces, saliva, cerebrospinal fluid, or blood, that is examined or tested for the presence of microorganisms
microorganisms associated with a certain area of the body without causing diseases
cultures composed of cells arising from a single progenitor
free of any microbial contaminants
origins of colony forming unit
1. single cell
2. cluster of cells
survival structure that can create a new viable cell
What are the two types of isolation methods?
streak plate; pour plate
Who developed the streak plate and pour plate technique?
Technique where sterile inoculating loop is used to spread inoculum across the surface of a solid medium in Petri dishes and the loop is used to lightly streak a set pattern that gradually dilutes the sample to a point that CFUs are isolated from one another
streak plate technique
Clear, flat culture dishes with loose-fitting lids
Technique used when CFUs are separated from one another using a series of dilutions
a gelling agent derived from the cell walls of algae
What is observed in inspection and identification?
shape, margin, elevation, size, texture, appearance, pigmentation/color, optical property
tools used for identification
macroscopic and microscopic appearance; biochemical tests; genetic characteristics; immunological testing
look at capabilities of microorganisms
to look at strain or cause of outbreak of disease
genetic characteristics (DNA)
testing of reactivity; what antibodies do or do not react to certain microorganisms
culture that contains one type of organism -- example?
pure culture ; lab
culture that has more than one type of organism --example?
mixed culture; environment
culture that has unwanted organisms growing-- example?
during inoculation- trying to not allow contamination
potentially hazardous cultures and specimens are usally disposed of by
steam sterilization or incineration
burning everything up
material supporting the growth of a microorganism
media in which the exact chemical composition is a known sample
growth media can be what forms of matter
solid or liquid
in growth media what is a commonly used solidifying agent
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