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Chem Quiz

Terms in this set (16)

Spectrophotometry
Light in a Narrow Wavelength Range
Bichromatic Analysis
Light Absorbance at 2 Different Wavelengths
Fluorometry
Light Emitted from Compounds that Absorb Electromagnetic Radiation. Become Excited and return to energy states slightly lower than their Original energy states
Turbitity
Light Blocked by particulate matter as light passes thru the cuvette by a spectrophotometer
Nephelometry
Light Scattered by small particles at Right Angles to the Beam incident to the cuvette
Osmometry
Total and Number of Dissolved Particles in a solution based on colligative properties ( freezing point depression and vapor point depression most commonly measured by instruments)
Chromatography
Separation of mixtures into Individual Components based on specific differences in Physical Characteristics
Retention Factor Rf
Distance a component migrates compared with the distance the solvent moves
Electrophoresis
Separation of proteins Based on ionic charge & molecular size
Poteniometry- pH meters,
Ion selective electrodes (ISE)
Potential (voltage) Difference between reference and indicator electrodes when NO current is passing thru the cell
Coulometry
Titration in which the titrant is Electrochemically generated
Amperometry
Current flowing thru an electrochemical cell when a constant electric potential is applied to the Electrodes
Immunoassay
Utilize antigen and antibody reactions
Immunoelectrophoresis (IEP), Immunofixation Elctrophoresis (IFE)
Protein measurement in serum and urine
Enzyme linked immunosorbent assays (ELISA)
Heterogeneous immunoassay-technique where it is necessary to physically separate labeled antigen or hapten bound to antibody from a labeled antigen or hapten free in solution
Enzyme multiplied immunoassay technique
(EMIT)
Homogenous immunoassay-technique that DOES NOT require the physical separation of the bound and free labeled antigen
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