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PCC BY 206 Lab Final
Terms in this set (61)
What is added to the Bile Esculin Agar to separate the Streptococcus bovis group and enterococci from other streptococci?
Medium is darkened within 48 hours
What shows a positive test for the Bile Esculin test?
Bile Esculin Test
What test do we use to determine Group D Streptococcus or Enterococcus?
Hektoen Enteric Agar/Test
What test do we use to isolate Salmonella and Shigella
lactose, sucrose, salicin
Hektoen Enteric Agar is based on the ability to ferment what 3 sugars?
Organism produces acid from lactose fermentation
What does a pink to orange growth result determine on the Hektoen Enteric Agar?
What converts hydrogen peroxide into water and gaseous oxygen?
What test uses hydrogen peroxide?
Visible bubble production
What indicates a positive test for the catalase test?
What test is used to identify organisms that produce the enzyme catalase?
What type of bacteria contain the enzyme nitrate reductase, performing a single-step reduction of nitrate to nitrite?
What is the multi-step process which allows bacteria to enxymatically convert nitrate to molecular nitrogen (N2)?
Nitrate Reduction Test
In what test do we use the Durham test tube?
By a visual inspection for the presence of gas in the Durham tube
How do we examine for evidence of denitrification?
sulfanilic acid (nitrate reagent A) and naphthylamine (nitrate reagent B)
In the Nitrate Reduction Test, if no visible evidence of denitrification takes place, what are added to the medium to test for nitrate reduction to nitrite?
In the Nitrate Reduction Test, a formation of what color after the addition of reagents indicates that the organism reduced nitrate to nitrite?
The nitrate either was not reduced or was reduced to one of the other nitrogenous coumpounds.
In the Nitrate Reduction Test, what does it mean if there is no color change after the addition of reagents?
In the Nitrate Reduction Test, what is the final component we add to the broth to catalyze the reduction of any nitrate to nitrite?
A red color
What is a positive result of the Nitrate Reduction Test?
Inspecting the Durham tubes for an indication of gas production.
Whats a preliminary step before completing the Nitrate Reduction Test?
The organism reduced nitrate to something other than nitrite.
Organism does not reduce nitrate.
In the Nitrate Reduction Test, what does it mean if the tube did not change colors to red after the addition of zinc?
Changes to Red?
Virtually, all members of what class perform a one-step reduction of nitrate to nitrite?
Dentrification-production of nitrogen gas (NO3 -> NO2 -> N2)
In the Nitrate Red. Test, whats the interpretation of gas (nonfermenter)?
Requires addition of reagents
What steps would you take in the Nitrate Reduction Test after the formation of gas (fermenter, or status is unknown)?
What is an enzyme that some bacteria produce to hydrolyze the milk protein casein, the molecule that gives milk its white color?
What type of agar do we use with the Casein Hydrolysis Test to identify casease-positive organism?
A clearing in the agar; Casease is present
What is a positive test for the Casein Hydrolysis Test and what does it mean?
In the Casein Hydrolysis Test, which bacteria had clearing on the agar?
What is an enzyme that catalyzes the depolymerization of DNA into small fragments?
blue-green color (because it contains methyl green dye)
What color is the agar used in DNA hydrolysis test?
Casein Hydrolysis Test, DNA Hydrolysis Test, Bacitracin/Novobiocin/Optochin Test
With what 3 tests are indicated positive by a clearing around the agar?
DNA Hydrolysis Test
What test is used to distinguish Serratia species from Enterobacter species, Moraxella catarrhalis from Neisseria species, and Staphylococcus aureus from other Staphylococcus species?
Red color formation
What is a positive test result for the PYR test?
Organism produces L-pyrrolidonyl arylamidase
What does a positive test result for the PYR test indicate?
What test is designed for the identification of group A streptococci (S. pyogenes) and enterococci?
What antibiotic is effective only on bacteria that have cell walls and are in the process of growing?
Bacitracin, Novobiocin, and Optochin Susceptibility Test
What test are used to identify B-hemolytic group A streptococci (Strep. pyogenes) from other B-hemolytic streptococci? Also, Staph from Micrococcus and Stomatococcus.
Bacitracin Test ~ zone of clearing?
Novociocin Test ~ zone of clearing?
Optochin Test ~ zone of clearing?
Which antibiotics interfere with the production of ATP?
Blood agar (sometimes called sheep blood agar because it contains 5% sheep blood)
What type of agar do we use for Bactiracin, Novobiocin, and Optochin Test?
What test is used for isolation and cultivation of many types of fastidious bacteria?
What are substances that are capable of destroying RBCs and hemoglobin?
What type of hemolysin is the total destruction of RBCs and hemoglobin, resulting in a clearing of the medium around the colonies?
Which hemolysin is the partial destruction of RBCs and produces a greenish discoloration of the agar around the colonies?
Which hemolysin is actually non-hemolysin and appears as simple growth with no change to the medium?
Hemolysins produced by streptococci are called what?
In which test do we stab the agar 2x?
Tube Test & Slide Test
What are the 2 types of the Coagulase Test?
Tube Test; Slide Test
Which type of the Coagulase test detects bound OR free coagulase? ONLY bound coagulase?
Coagulation of the rabbit plasma within 24 hours
Whats a positive result for the Tube Test (Coagulase Test) ?
How long does it take to see a result with the Slide Test (Coagulase Test) ?
Red diffuse growth radiating outward from stab line (Organism is motile).
What indicates a positive result for the Motility Test?
Antimicrobial Susceptibility Test
In which test do we use McFarland turbidity test?
What test is also called disk diffusion test?
What are natural antimicrobial agents produced by microorganisms?
Which test will test the susceptibility of E. coli and staph. aureus to penicillin, chloramphenicol, trimethoprim, and ciprofloxacin?
Staph. aureus and S. epidermidis
What 2 bacteria are notorious for forming biofilms on invasive medical devices resulting in nosocomial infections?
What stain did we use to stain the the biofilm?
What bacteria showed a positive test in the DNA Hydrolysis Test?
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