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Oxidative Phosphorylation Pogil
Terms in this set (35)
Where is the electron transport chain located?
within the inner mitochondrial membrane
In the electron transport chain, where is the highest concentration of hydrogen ions located?
intermembrane space (between inner and outer membranes)
How do hydrogen ions reach the intermembrane space?
they diffuse through embedded protein channels via facilitated diffusion
Why is energy required to move the hydrogen ions across the membrane into the intermembrane space?
the ions are moving against their concentration gradient, from low to high concentration
What molecules carry the high potential energy electrons used to pump hydrogen ions across the inner mitochondrial membrane?
NADH and FADH2
Where did the electron acceptor molecules (NADH and FADH2) come from?
they're produced in glycolysis, the link reaction, and the Krebs cycle (link reaction may be considered part of the Krebs cycle)
When electrons are released from the electron acceptor molecules, what else is produced?
Is the release of an electron from an electron acceptor molecule oxidation or reduction?
What molecule is the final electron acceptor after the electron has moved through the electron transport chain?
What compound is formed as a final product of the electron transport chain?
Is any ATP produced in the electron transport chain?
Is any ATP used in the electron transport chain?
Describe the movement of hydrogen ions through the membrane during chemiosmosis.
the hydrogen ions move from a high concentration in the intermembrane space to a low concentration in the mitochondrial matrix through ATP synthase via facilitated diffusion
Would free energy be required for the hydrogen ions to move from the intermembrane space to the mitochondrial matrix?
No, because they're moving from high to low concentrations
What is the name of the embedded protein that provides a channel for the hydrogen ions to pass through the membrane?
The flow of hydrogen ions through the protein channel provides free energy to do work. What process in chemiosmosis requires energy?
phosphorylation (ADP --> ATP)
During oxidation, what molecule is being phosphorylated?
Under ideal conditions each NADH molecule will result in 3 ATP molecules, and each FADH2 molecule will result in 2 ATP molecules during oxidative phosphorylation. Calculate the total number of ATP molecules that might be produced in this phase of cellular respiration from one glucose molecule.
Considering all the stages of cellular respiration (glycolysis, link, Krebs cycle, and oxidative phosphorylation) how many ATP molecules are produced from one glucose molecule, assuming ideal circumstances?
glycolysis = 2
link = 0
Krebs = 2
oxidative phosphorylation = 34
Because of its role in aerobic respiration, oxygen is essential for most living things on Earth. Describe the role of molecular oxygen in aerobic respiration.
oxygen is an H+ ion acceptor through the electron transport chain which produces H2O and also helps to recycle NAD+ and FADH2 through the electron transport chain
In which phases is glucose used or produced?
In which phase is oxygen used or produced?
In which phase is carbon dioxide used or produced?
Krebs cycle and link reaction
In which phase is water used or produced?
In which phases are the 38 ATP used or produced?
oxidative phosphorylation, Krebs, and glycolysis
Where in the cell is glucose used or produced?
Where in the cell is oxygen used or produced?
Where in the cell is carbon dioxide used or produced?
inner mitochondrial matrix
Where in the cell is H2O used or produced?
mitochondrial matrix and inner mitochondrial membrane
Where in the cell is the 38 ATP used or produced?
mitochondrial matrix, cytoplasm, and inner mitchondrial membrane
Substrate level phosphorylation is the term used for phosphorylation that removes a phosphate from one molecule and joins it to another molecule. Oxidative phosphorylation is the term used for the attachment of free inorganic phosphate to a molecule. Identify the phases of cellular respiration that use substrate level phosphorylation and that use oxidative phosphorylation.
Glycolysis and the Krebs cycle use substrate level phosphorylation, and oxidative phosphorylation uses oxidative phosphorylation.
Which side of the inner mitochondrial membrane would have a higher pH?
During glycolysis the enzyme hexokinase uses ATP to transfer a phosphate to glucose to form fructose-diphosphate. Suppose that a cell has only glucose available for energy and that the activity of hexokinase is suddenly stopped. Explain in detail what is most likely to occur in the cell.
the cell wouldn't produce any ATP because glycolysis would not occur
Prokaryote cells must have energy for cellular processes just like eukaryote cells do. Yet, they have no mitochondria. Which phase(s) of cellular respiration would be unaffected by the lack of mitochondria in a cell?
The link reaction and Krebs cycle occur in the cytoplasm of prokaryotes in the same way that they occur in the mitochondria of eukaryotes. However, a concentration gradient across a membrane is a requirement of the electron transport chain. Propose an alternative site for that phase of cellular respiration in prokaryotic cells.
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