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How to perform test?

inoculate lactose broth with inoculating loop

Properly it test for

bacterias ability to ferment lactose

Media and reagents used:

lactose broth contains beef extract, gelatin peptone, and lactose. A phenol red indicator is added to indicate acid production from fermentation

pos. results

yellow after several drop of phenol red is added (indicating lactose fermentation)

neg results

reddish color

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