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Biochemical Tests

Terms in this set (81)

catalase enzyme
The Catalase Test tests for the presence of _____.
hydrogen peroxide
broken down by bacteria that produce catalase to form bubbles
bubbles
positive result of the catalase test
no bubbles
negative result of the catalase test
sugar fermentation and gas production
The Phenol Red Test tests for _____.
sucrose, lactose, glucose
sugars used in the Phenol Red Test
phenol red
indicator in the Phenol Red Test
durham tube
small tube placed inside the test tube that indicates gas production in the Phenol Red test
yellow, is, is
The +/+ result of the Phenol Red Test is _____, sugar _____ fermented, and gas ______ produced.
yellow, is, is not
The +/- result of the Phenol Red Test is _____, sugar _____ fermented, and gas _____ produced.
red, is not, is not
The -/- result of the Phenol Red Test is _____, sugar _____ fermented, and gas _____ produced.
does not occur
The -/+ result of the Phenol Red Test is _____, sugar _____ fermented, and gas _____ produced.
decarboxylase
The Decarboxylase Test tests for the presence of _____.
decarboxylase
enzyme that removes the COOH group from an amino acid
dextrose
sugar fermented by all bacteria in the Decarboxylase Test
bromocresol blue
indicator in the Decarboxylase Test
purple
color of the Decarboxylase Test at high pH (basic)
yellow
color of the Decarboxylase Test at low pH (acidic)
anaerobic
type of environment required for the decarboxylation of amino acids
immersion oil
used to create an anaerobic environment in the Decarboxylase Test
lysine, arginine, ornithine
amino acids used in the Decarboxylase Test
purple
positive result of the Decarboxylase Test
yellow
negative result of the Decarboxylase Test
citrate utilization
The Simmon's Citrate Test tests for _____.
citrate
only source of carbon in the Simmon's Citrate Test
bromothymol blue
indicator in the Simmon's Citrate Test
blue
color of bromothymol blue at pH above 7.6 in Simmon's Citrate Test
green
color of bromothymol blue at neutral pH in Simmon's Citrate Test
blue
positive result of Simmon's Citrate Test
green
negative result of Simmon's Citrate Test
gelatinase
The Gelatin Test tests for the presence of _____.
gelatinase
enzyme derived from collagen and found in connective tissue
solid
state of gelatin at < 37 degrees
liquid
state of gelatin at > 37 degrees
liquid after refrigeration
positive result of the Gelatin Test
solid after refrigeration
negative result of the Gelatin Test
sulfur reduction, fermentation, gas production
The TSI Test tests for _____.
glucose, lactose, sucrose
carbohydrates found in the TSI Test
carbon, nitrogen
animal proteins found in the TSI Test
ferrous sulfide and sodium thiosulfate
sulfur sources in the TSI test
phenol red
pH indicator in the TSI Test
ferrous sulfate
hydrogen sulfide indicator in the TSI Test
glucose only fermenter
TSI Test result with low pH, yellow medium at first, reversion after 12 hours, and red slant and yellow butt
glucose and lactose/sucrose fermenter
TSI Test result that is yellow throughout, produces gas, has fissures in the medium, and lifting occurs
nonfermenter
TSI Test result that uses peptone and amino acids, alkalinizes the medium, turns red, and is an obligate aerobe with a red slant
black
color that indicates sulfur reduction and fermentation in the TSI Test
glucose only fermenter
TSI Test result with a red slant and black butt
glucose and lactose/sucrose fermenter
TSI Test result with a yellow slant and black butt
nonfermenter
TSI Test result indicated by red over red
glucose fermenter
TSI Test result indicated by red over yellow
glucose and lactose/sucrose fermenter
TSI Test result indicated by yellow over yellow
glucose fermenter and sulfur reducer
TSI Test result indicated by red over black
nonfermenter and sulfur reducer
TSI Test result indicated by yellow over black
butt
stabbed during TSI Test
slant
fishtailed during TSI Test
black
positive result of sulfur portion of SIM Test
tan
negative result of sulfur portion of SIM Test
tryptophanase
The SIM Test tests for the presence of _____.
indole
tryptophan -> pyruvate + ammonia + _____
Kovac's reagent
reacts with indole during SIM Test
red
positive result of indole portion of SIM Test
yellow
negative result of indole portion of SIM Test
SIM and TSI
tests that use a stabber instead of a loop
radiating growth or turbidity
positive result of motility portion of SIM Test
straight growth
negative result of motility portion of SIM Test
gram positive
inhibited by Bile Esculin Test
gram negative
favored by Bile Esculin Test
beef extract, digest of gelatin, esculin, bile, ferric citrate
ingredients of Bile Esculin Test
esculin
glycoside composed of glucose and esculetin
beef extract and gelatin
source of nutrients during Bile Esculin Test
bile
selective agent in Bile Esculin Test
ferric citrate
source of oxidized iron in Bile Esculin Test
ferric citrate
indicator in bile Esculin Test
brown
positive result of Bile Esculin Test
tan
negative result of Bile Esculin Test
starch
large carbohydrate consisting of an abundant amount of glucose
amylase and glucosidase
exoenzymes that break down starch in bacteria
beef extract, starch, agar
ingredients in the Starch Test
iodine
added to detect the presence or absence of starch in the Starch Test
clear
positive result of the Starch Test
brown
negative result of the Starch Test
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