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POGIL: Gene Expression - Transcription (for Dr. Smasho's surprise attack)
UPDATE: You can use your POGIL on the quiz
Terms in this set (27)
Where the DNA is found in the cell
Where cell transcription takes place
The polymer synthesized during transcription
Monomers used to construct mRNA and where they are found
Free RNA nucleotides, cytoplasm
Enzyme (protein) required for transcription
Base-pair rule for a DNA strand matching an RNA strand
GC, AT, AU
RNA+DNA base-pair rule compared to DNA+DNA base-pair rule
RNA+DNA has AU because RNA has Uracil
Strand in DNA containing the "blueprint" for the pre-mRNA
Direction DNA molecule reads
3 prime to 5 prime
Direction the pre-mRNA molecule is constructed
5 prime to 3 prime
Explanation of why the process in Model 1 is called transcription
Makes an exact copy of information in DNA for RNA
Parts making up the transcription initiation complex
Transcription factor proteins, RNA polymerase
Where the transcription initiation complex forms on the DNA strand
Is it likely that a cell would transcribe all the genes within its nucleus simultaneously?
No - cell only makes genes as needed to make specific proteins
Is it likely that all cells transcribe all their genes at some point in their lifetime?
No - genes are switched on and off depending on location and function of the cell.
Removed from the pre-mRNA to make it mRNA
Added to the mRNA (not in pre-mRNA), locations
Methyl cap at 5 prime, Poly-A tail at 3 prime
mRNA leaves the nucleus through the
Starting point for the polypeptide "blueprint"
Start codon - AUG
m in mRNA is short for messenger. WHY????
Carries blueprints like a messenger (and like a boss)
Human genome produces about 25,000 genes, but 100,000 different polypeptides. EXPLAIN????????
Single sequence of pre-mRNA produces different mRNA sequences depending on what introns are removed
Purpose of the Poly-A tail
Prevents info-carrying part of mRNA from being destroyed by exonucleases before a polypeptide can be formed (they're the sacrifices to the exonucleases gods)
Explain why some Poly-A tails are shorter than others
The proteins are needed for a shorter amount of time:
Short tail = short lifespan = briefly needed proteins
Steps of Transcription
*Pre-mRNA made from template strand of DNA (starts at promoter by transcription initiation complex: RNA polymerase + transcription factors)
*RNA polymerase reaches terminator - pre-mRNA is released
*Introns removed, methyl cap + poly-A tail added
RNA IS BORN
Exonuclease is this type of biological molecule
Free Nucleotides come from
Cytoplasm - from mRNA hydrolyzed by exonucleases
*All species use same nucleotides in DNA and mRNA
*All species use promoter region to initiate transcription
*All species use similar RNA polymerase enzyme
THIS SET IS OFTEN IN FOLDERS WITH...
Gene Expression- Translation POGIL
Control of Gene Expression in Prokaryotes
Genetic Mutations POGIL
Genetic Mutations POGIL
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