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BIOL 101 Enzyme Lab
Rob Swatski, Associate Professor of Biology at HACC York Campus (HACC, Central Pennsylvania's Community College) http://robswatski.virb.com/
Terms in this set (36)
a large globular protein ranging in molecular weight from 10,000 to 500,000 Daltons, which accelerates and regulates biochemical processes
substance that speeds up the rate of a chemical reaction
True or False? Chemical reactions in living cells are much faster than reactions in most other systems.
the basic building block of protein molecules
The average amino acid weighs approximately ___?
covalent bond formed between amino acids
depressions found on the three-dimensional shape of an enzyme
the part of an enzyme where the chemical reaction occurs
the reactant of an enzyme-catalyzed reaction
enzyme substrate complex
this is the temporary union between the substrate and the active site of an enzyme
enzyme product complex
this is the temporary union between the products made as a result of an enzymatic reaction and the active site of an enzyme
True or False? Although they can cause chemical
changes in other molecules, enzymes are themselves unchanged by the reaction.
One enzyme can carry out ___ of reactions in a minute.
a plant enzyme that converts catechol to benzoquinone
a colorless phenolic compound common in plant cells
the brown pigment that catechol was converted into during the experiment
The familiar browning of cut apples and potatoes is due to the production of benzoquinone in the presence of ___ and catechol oxidase.
chemicals that must bind to the enzyme for it to be functional
non-protein substances that usually bind to the active site on the enzyme and are essential for the enzyme to work; an example is a metal ion like copper (Cu+)
chemicals that shut off enzyme activity
What is a major source of human error in the enzyme experiment?
cross-contamination of pipettes
any substance that mimics the substrate of an enzyme and thus inhibits its activity
Which pseudosubstrate did we use in the enzyme experiment?
this chemical combines with the copper in catechol oxidase and inhibit its enzyme activity
this occurs when a molecule that is structurally similar to the substrate for a particular reaction competes for a position at the active site on the enzyme
this occurs when the inhibitor binds to a part of the enzyme that is not the active site
Adding additional substrate will reverse ___ inhibition.
Is PTU a competitive or noncompetitive inhibitor?
noncompetitive inhibitor, because adding extra substrate did not reverse the inhibition
True or False? Changes in the concentration of hydrogen ions will have no effect on the function of an enzyme.
a substance that increases the hydrogen ion concentration of a solution
a substance that decreases the hydrogen ion concentration of a solution
a compound which can bind or release hydrogen ions and therefore will maintain a stable pH even if some extra acid or base is added to a solution
a protein that cannot function properly because it's shape has been significantly altered by changes in temperature or pH
The rate at which chemical reactions take place is largely determined by the ___ of the environment.
Generally, for every 10 degree Celsius rise in temperature, the reaction rate ___.
the specific temperature and/or pH where enzyme activity is maximized
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