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28 terms

MCC BIO 205 Study Guide Lab Practical

EX. 12,14,15,16,19.
STUDY
PLAY
Macroscopic and microscopic appearence of mold
Macroscopic- Moist fungal hyphal filaments intertwined in to a mass of fuzzy or hairy, colorful colonies.
Microscopic-multicellular filaments called hyphae
Macroscopic and microscopic appearence of yest
Macroscopic- Colonial appeareance is usually moist, creamy and white in color although a few yeast can produce pigment.
Microscopic- Yeast are round to oval in shape and usually 5-10X larger that bacteric
Common sporulation patterns
...vegetative and aerial hyphae
Identify mold based on its macroscopic and microscopic sporulation characteristics
...sporangiospore and conidiospore
Bacteriophage
Viruses that infect bacteria
lytic
when burst open their bacterial host
plaque
bacteria area of clearing
purpose for making dilutions of the T-4 virus
In order to calculate the number of phages in a sample
calculate the titer of phage given the number of plaques and the dilution of the plate.
Plaque count X Dilution factor X Sample amount= PFU's/ml of sample
230 X 10^-6 X 10^-1= 2.3 X 10^9
Staphylococcus catalase test
Immediate appearence of bubbles indicates the organism can produce catalase.
streptococcus catalase test
No bubbles, organism does not produce catalase
Principle & state the reagent/media uses in catalase test
...Hydrogen proxide introduced to bacterial smear produces an immediate appearence of bubbles indicates the organism can produce catalase.
Principle & state the reagent/media uses in coagulase test
rabbit plasma is mixed with bacteria. Obvious clumping of bacteria indicates that organism produces bound coagulase
Differentiate the 2 species of staphylococcus by coagulase test and reaction to Mannitol Salt agar
S.aureus- ++
S. epidermidis- --
How is Mannitol salt agar both selective and differential media
...
Determine the species of micrococcus by pigmentation
M.roseus- rose pigmented
M. luteus-yellow pigmented
Determine the species of micrococcus by nitrate test results
M.roseus- Positive
M. luteus- Negative
recognize different types of hemolysis of organisms growing on blood agar
Alpha- Partial, green, old bruse
Beta- Complete, clear,transparent all the way through
Gamma- no hemolysis, surface growth
why is streak-stab method used for blood agar inoculations
to enhance the appearance of hemolysis
state the atmosphere used to grow streptococcus
Candle jar for microaerophilic environment at 30 degrees C
Identify the Alpha Streptococcus given the appropriate biochemical test
...
Identify the Beta Streptococcus given the appropriate biochemical test
...
Differentiate the Enterococcus from other alpha hemolytic streptococcus
Positive bile esculin hydrolysis
How does CNA agar aid in the identification of bacteria in a mixed unknown
Colistin and naladixic acid are two antibiotics that supress the growth of gram-negative organisms, there for selective for gram-positive organsims. differential by hemolyzing red blood cells
How does MAC agar aid in the identification of bacteria in a mixed unknown
selective for gram-negative organisms since gram-positive oragnisms are unable to tolerate the dyes and the bile salts that are in the media. It is differential because of the lactose in it.
Differentiate bacteria that grow on MAC plate by their ability to ferment lactose
Positive pink indicator lactose fermenter- Staphylococcus
Negative- collorless or yellow- Micrococcus
Identify gram negative rod by flow chart (dichotomous key) & biochemical test reactions
...
Construct a flow chart by biochemical reactions someone could follow to identify bacteria
...