PLPA Lab Practical

PPE for every lab
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Terms in this set (31)
PPE for every lab
lab coat, safety goggles, and gloves
sterile technique
don't breathe in area, always keeping culture under bunsen burner and sterile hood
sterilization methods
flame and chemical sterilization, autoclave, and sterile
selective media
media adjusted to allow for the growth of specific organisms while inhibiting the growth of others.
-grow specific bacteria
differential media
are used to distinguish between the target organism and all others that may grow on that particular medium.
-biochemical reactions
-change colors
dissecting microscope
are used to observe relatively large objects, at magnifications of 10-40X and sometimes up to 100X.
-incident light (top lighting)
-normal microscope
compound microscopes
use multiple lenses to observe smaller specimens that are placed on microscope slides.
-transparent/translucent specimens
magnification
a measure of the ability of the microscope to enlarge an image.
resolution
is a measure of the ability of the microscope to separate different points of the image
Koch's Postulate
1. The suspected causal agent must be present in every diseased plant examined.

2. The suspected casual agent must be isolated from the diseased host and growth in pure culture.

3. When a pure culture of the suspected casual agent is inoculated into a healthy susceptible host, the host must produce the specific disease.

4. The same casual agent must be recovered again from the experimentally inoculated and infected host.
Technique for transferring bacteria to a plateThe Quadrant StreakGram Positivestain purple and retain crystal violetGram Negativeare not stained by crystal violet and change colors to pink.Scientific Name of Root Knot NematodeMeloidogyne incognitaSymptoms of Root Knot Nematode-root gall formation -plant stunting -stubby root formation -dead ares within roots -excursive branching of roots -death of entire root branchFemale Nematodesare the most prevalent sex in populations and care the most damage to the host plants.Inoculation of Root Knot Nematodesinjecting egg solution into plant with needle at the base and covering with soilAgrobacterium Tumefaciens Signs/Symptoms1. causes crown galls on dicots 2. acetosyringone attracts and activates oncogenic potential 3. soil borne microbe 4. does the transfection thingInoculation technique for A. tumefaciensswabbing, stabbing, spraying, injecting, mechanical transmission, and soil amendmentBroad groups of fungioomycetes zygomycetes ascomycetes basidiomycetesoomycetesoospores (sexual) zoospores (asexual)zygomycetessporangiospores (asexual)ascomycetesascospores (asexual)basidiomycetesbasidiospore (sexual)PCR components-template DNA -DNA polymerase -nucleotidesreasons why oomycetes are not considered true fungicoenocytic, produce motile spores, contain cellulose in cell wallsystematic infectionsvirus spreads throughout the plant and symptoms include mosaic, mottling, stunting, leaf curling, leaf wrinkling and yellowing of tobacco leaves.local infectionslocated infections in the inoculation areas. symptoms: necrotic spotsSusceptible Plant Host to TMVtobacco, tomato and many other cropsComponents of ELISA-substrate -antibody enzyme -conjugate antigen -virus specific antibodyELISAEnzyme, Linked, ImmunoSorbent, Assay