Upgrade to remove ads
HT LAB lecture 1 Midterm TEXT. COMPLETE. NOT DOUBLE CHECKED, HT LAB lecture 2 Midterm TEXT. COMPLETE. NOT DOUBLE CHECKED. (Lab W6 podcast end 32:10), HT LAB lecture 3 Midterm TEXT. COMPLETE. NOT DOUBLE CHECKED (Lab W6 podcast end 32:10)
Terms in this set (51)
which part of blood smear is too thin to be examined under microscope?
at feathered edge.
too thin because the blood cells may incorrectly look like spherocytes
how know if area is too thin to examine?
1. cannot see central pallor of RBC
2. too many gaps
3. RBCs look too spherical
how know if at right area to examine?
1. see nice central pallor AKA pale area of RBCs
2. the cells have very little to no overlap
how can you tell if RBC is small or big?
1. compare it to a lymphocyte nucleus in the slide. a typical RBC is size of lymphocyte nucleus.
if RBC is smaller than lymphocyte nucleus
= RBC is small
monocyte looks like
= kidney shaped
=abundant pale blue. low nuclear to cytoplasmic ratio.
= largest of the WBC
lymphocyte looks like
= large spherical. has dense chromatin.
=thin rim of pale blue with NO granules.
= its nucleus is size of RBC
neutrophil looks like
= has pale red & blue cytoplasmic granules
eosinophil looks like
= has red, orange cytoplasmic granules
basophil looks like
= bilobed. obscured by the granules
= has purplish-black cytoplasmic granules. low nucleus to cytoplasm ratio
platelet looks like
looks like "dust bunnies" circulating around. shows no defined nucleus.
can be small to large (large= size of RBC)
platelet the size of RBC
list WBC from most to least abundant
how to distinguish between basophil and lymphocyte
= has a lot of dark granules. use fine focus to see them.
makes up polymorphonuclear leukocytes
neutrophil with 3 to 5 lobes
should you use just the number of lobes of nucleus to determine WBC?
Should look at the WHOLE cell, not just the nucleus lobes, because some WBC that are typically bilobed may be segmented
desc. what erythroblast looks like
1. , fine chromatin pattern, usually 1 to 2 Nucleoli visible in the nucleus. Nucleoli are larger than those found in the myeloblast.
2. high N: C ratio. basophilic AKA stains blue
MEM: when the nucleus was BLASTed by a bomb, it looked, there was RED all over the place and the nucleus looked like NUCLEOLI!
3. 14-24 um
list order of maturation stages of RBC
2. basophilic normoblast
3. polychromatophilic normoblast
4. orthochromatic normoblast
6. mature RBC
1. nucleoli usually not visible if present. Nucleus has a slightly coarser chromatin pattern than in the previous stage
2. cytoplasm is intensely basophilic. high N:C ratio
self note: the cytoplasm looks like a super thin ring around the nuclear region
MEM: BASically that cytoplasm looks like a RING that I want someone to just propose to me with already!!
desc. what polychromatophilic normoblast looks like
1. smaller nucleus than basophilic normoblast. Nuclear chromatin is thicker and irregularly condensed.
2. Have relatively more cytoplasm than basophilic normoblast. . The cytoplasm contains a varying mixture of pink due to hemoglobin and blue due to RNA
MEM: POLY was a cute bird that FILLED everyone with enjoyment because she was more LIGHT and PINKISH colored, which people liked to see
desc. what orthochromatic normoblast looks like
1. clumped pyknotic (lacking parachromatin-white areas) features
2. Cytoplasm is predominantly pink
MEM: the ORTHOpedics patients lacked WHITE cast material, so everyone was panicking!
Also imagine an "O" (O for orthochromic) that has purse strings that are puckering up
desc. what polychromasia looks like
2. lack of central pallor. Diffusely basophilic
3. 8-10 um
MEM: POLY the bird from ASIA was jealous of the other POLIES because she lacked central pallor. She felt a A LITTLE BIT BLUE (not completely blue, hence the mix of red and blue)
desc. morphology of Spherocyte
no central pallor
desc. morphology of hereditary Spherocytosis
all RBC are spherocytes (unless they are being treated)
howell- jolly bodies
basophilic nuclear remnants of DNA (clusters of DNA)
MEM: dog was HOWLing becuase wish he could take a DNA test to figure out who his real father is
prussian blue stain stains _____ blue on and therefore is seen in what types of inclusions
2. pappenheimer bodies
desc. the RBC with IDA morphology.
* must describe 2 key things about it!!
1. hemoglobin content
= hypochromic= more than 1/3 central pallor
= microcytic= smaller than lymphocyte nucleus
desc. morphology of RBC with folate or B12 deficiency .
2. type of WBC seen
2. neutrophils will be hypersegmented
3. Howell-Jolly bodies, since DNA synthesis is affected
a MACROscale of people are DEFICIENT in food , and so was HYPER about getting NEW food supply. They practically HOWELLED for new food!
Deficiency= 0= Ovalocyte!
what type of CBC results are expected of megaloblastic anemia?
2. high MCV
how do schistocytes form
cut by fibrin
morphology of echinocytes
1. central pallor
2. even distribution of projections
MEM: E= Even spiny projections.
think E= EARTH, which is CENTRAL to my PALs living with me in this world!
morphology of Acanthocytes
1. NO central pallor
2. UNeven distribution of projections
MEM= A is prefix for NO= NO central pallor.
A is also prefix for UN= UNeven distribution of projections
disease state that has acanthocytes
what can cause RBC membrane defects?
1. G6PD deficiency
2. being cut with fibrin strands
3. Hemolytic Disase of Newborn: baby is Rh positive, Momma is Rh negative and made Rh antibodies (made after 1st pregnancy)
desc. morphology of Thrombotic Thrombocytopenic Purpura (TTP)
cause: platelets are consumed by the abnormal clotting
1. RBC fragmentation occurs as a result of the clotting
2. Thrombocytopenic = low platelets
desc. morphology of Idiopathic (Immune) Thrombocytopenic Purpura (ITP)
1. NO RBC fragmentation
2. low platelets
MEM: that IDIOT forgot to bring the FRAGMENTED piece of RED roses. now we lost one million dollars in business!
patient presents with blood that has many spherocytes.
What could have caused it?
MEM: when the guys was in a FIRE, he bundled up in a SPHERE to protect his head. thankfully he survived and only suffered SEVERE BURNS
desc. morphology of thalassemia
1. poikilocytosis= high number of immature abnormally shaped red blood cells found in the bloodstream as a result of being prematurely released from the bone marrow
desc. lab findings of thalassemia
Lab findings include:
- hypochromic, microcytic anemia
- marked anisocytosis and poikilocytosis
- schistocytes, ovalocytes, and target cells
- basophilic stippling from α chain precipitation - increased reticulocytes and nucleated RBCs
desc. morphology of Sickle Cell Disease RBC
1. many target cells
2. have SC crystals= looks like "bird in flight"
3. cells look like a "boomerang" (self-term)
desc. morphology of "SC crystals"
looks like "bird in flight"
desc. morphology of teardrop cell
look like teardrops, many of which are pointed in different directions.
If ALL pointed in same direction
= is NOT teardrop cell. must redo slide preparation
patient has course basophilic stipling.
what could this be due to?
how to differentiate pappenheimer from Howell-jolly bodies?
pappenheimer bodies are smaller
howell- jolly bodies represent
heinz bodies represent
MEM: HEIZ ketchup is RED. HEMOGLOBIN makes blood RED
pappenheimer bodies represent
MEM: the IRON carrying guy was PAPPYing on other women at the gym. THat's why they nicknamed Mr.Heimer as Mr.PAPPENHEIMER
basophilic stipling represents
MEM: Harry makes STIPPLES as fast as RIBOSOMES make proteins!
= type of parasite that can cause malaria
morphology of falciparum gametocyte
1. sickle cell shape
2. shows nucleus
if see cell is shown in ring form, what is the disease
malaria, BUT the exact type
cannot be determined with sole information given
desc. morphology of Hemoglobin C (HbC) crystal
shaped like a pill (self note)
YOU MIGHT ALSO LIKE...
Red Blood Cell Maturation
White Blood Cell Structure and Characteristics
OTHER SETS BY THIS CREATOR
OSCE chief complaint abdominal pain. INCOMPLETE
OSCE chief complaint breathing trouble
OSCE chief complaint diplopia
OSCE chief complaint neck pain