How can we help?

You can also find more resources in our Help Center.

37 terms

Dermatology Lab Tests

STUDY
PLAY
Scabies Scraping
#15 blade
glass slide
Mineral Oil
Microscope
Scabies
burrow
Scabies
burrow
Scabies
burrow
Scabies
burrow
Scabies
Scabies
Scabies
Scabies
Bullous Pemphigoid
Bullous Pemphigoid
Immunoflourescence
Immunoflourescence
Special Stains - PAS for fungi
Special Stains - PAS for fungi
Special Stains - PAS for fungi
Herpes - Tzanck Smears
Herpes - Tzanck Smears
Herpes - Tzanck Smears
Herpes - Tzanck Smears
Collecting Skin Scrapings for Fungal KOH Exam
Select a scaly lesion
Use sterile #15 blade or glass slide to take skin scrapings from representative lesion
Scrape the skin scales into a single compact pile
Add 1 drop of 10-20% KOH Solution or Chlorazol Black E, cover with cover slip
Heat gently over butane burner, not alcohol flame, avoid boiling
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea Collecting Skin Scrapings for Fungal KOH Exam
What type of exam would you do?
Tinea(Pityriasis) Versicolor
malassezia globosa, and malassezia furfur
Tinea(Pityriasis) Versicolor
malassezia globosa, and malassezia furfur
Tinea(Pityriasis) Versicolor
malassezia globosa, and malassezia furfur
Tinea(Pityriasis) Versicolor
spaghetti and meatballs
malassezia globosa, and malassezia furfur
A fungal culture
____ can determine which type of fungus is present. Since fungi generally grow slowly, it can take 2- 4 weeks for a ____ to produce test results.
dermatophyte
About 90% of fungal toenail infections are caused by a type of fungus called a ?
Fungal Cultures
A few culture mediums are available for dermatophyte growth.
Sabouraud agar containing neopeptone or polypeptone agar and glucose is often used for fungal culture. However, it does not contain antibiotics and may allow overgrowth of ____ and bacterial contaminants.
Mycosel, a commonly used agar, is similar to Sabouraud agar but has antibiotics.
Commonly, dermatophyte test medium (DTM) is used. It contains antibacterial (ie, gentamicin, chlortetracycline) and antifungal (ie, cycloheximide) solutions in a nutrient agar base. This combination isolates dermatophytes while suppressing other fungal and bacterial species that may contaminate the culture
Following culture inoculation, potential fungal growth is monitored at 2 weeks and 4 weeks.
Positive culture results vary depending on the medium used.
DTM contains phenol red solution, which causes a color change from straw-yellow to bright-red under alkaline conditions, indicating a positive dermatophyte culture result. However, the color makes identification of culture morphology (particularly pigmentation) difficult.
Sabouraud or Mycosel agar should be used to assess gross and microscopic colony characteristics.
Fungal Cultures
Sterile cotton swab or #15 blade
Place scales on proper ____ media
DTM, Sabouraud or Nickerson's Media
Incubate at room temperature for 4 weeks, read culture at 2 and 4 weeks
Must be CLIA approved lab
PAS
What stain is used on a nail Biopsy for Fungus