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Mastering 16

Terms in this set (87)

To produce a DNA fragment that contains the whole gene and has two sticky ends, where must the enzyme's restriction sites be located?
outside the gene on both sides, but not inside the gene
Which enzyme(s) will produce a DNA fragment that contains the entire vgp gene (shown in red) and has "sticky ends"?
Select all that apply.
HindIII
BamHI

(Cloning the vgp gene requires an enzyme that has restriction sites on both sides of that gene but not within the gene. Two enzymes, HindIII and BamHI, satisfy that requirement, while also producing DNA fragments that have sticky ends. Sticky ends make it possible for the fragments to combine with the DNA of a cloning vector, such as a plasmid.)
For the first time, the U.S. Food and Drug Adminis
food from a genetically altered animal
The fish in the video have been genetically engineered to _____.
grow faster
The modified salmon were created by _____.
adding genetic material from a Pacific salmon and an eel-like fish
According to the producers of the genetically modified salmon, the meat _____.
looks and tastes the same as unmodified salmon
What concerns do some consumer groups have about genetically modified fish?
They want food from genetically modified fish to be clearly labeled as genetically modified.
They want to prevent genetically modified fish from breeding with wild fish.
They want more studies on the health effects that genetically modified fish may have on people who eat it.
How does the company raising these fish claim to prevent the genetically modified fish from breeding with wild fish?
The genetically modified fish are sterile.
Folk singer Woody Guthrie died of Huntington's disease, an autosomal dominant disorder. Which statement below must be true?
It is very likely that at least one of Woody Guthrie's parents also have had the allele for Huntington's disease.

(Unless the disease is caused by a new mutation, which is quite rare, individuals with a dominant condition must have inherited the dominant allele from one of their parents. As it happens, Guthrie's mother also died of Huntington's disease.)
Various procedures can be used to detect genetic disorders before birth. Among the tests discussed in this chapter, which is the least invasive (list first), and which two allow the chromosomes of the fetus to be examined?
ultrasound imaging ... chorionic villus sampling ... amniocentesis

(Both CVS and amniocentesis involve the preparation of a karyotype using fetal cells.)
In order to insert a human gene into a plasmid, both must _____
be cut by the same restriction enzyme

(Doing so will result in the formation of complementary sticky ends.)
What enzyme forms covalent bonds between restriction fragments?
DNA ligase

(DNA ligase catalyzes the formation of covalent bonds between restriction fragments.)
The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
sticky ends
The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____.
The figure shows the part of the DNA. One strand from left to right reads T G C A G A A T T C. The second strand, which is complementary to the first, reads backwards C T T A A G, excluding the first four nucleotides.
-ACGT
What information can not be obtained from the sequence of a gene?
Whether the gene is methylated.
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