77 terms

Clin Lab 1 Final

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Blood Rocker
Instrument in the lab used to keep blood tubes at an even distribution
Plasm
Fluid yielded when unclotted blood is centrifuged
Electron Microscope
Most powerful microscope that can magnify up to 250,000x
Serum
Fluid yielded when clotted blood is centrifuged
Anticoagulant
Keeps blood from clotting
Coarse Knob
The knob that brings the microscope into "rough" focus
Iris Diaphragm
Part of the microscope used to adjust the light intensity
Field
Viewable area when looking in the microscope
Refractometer
Can be used to measure Urine Specific Gravity and Total Plasma Protein
Bad Sample Handling
Can cause hemolysis and inaccurate results
Centrifuge
Instrument in the laboratory that separates the liquid from solids using high speed
Sodium Citrate
Anticoagulant that is the first choice for coagulation studies
30 Minutes
How long blood has to clot before you can spin it down
Catalyst One
Chemistry analyzer used in our lab
Lasercyte
Machine in our lab used to perform electronic cell counts
Red Top
Tube that should never be placed on a blood rocker after filled with blood
Red/Gray Mottled Tube
Serum Separator
Lavendar Tube
EDTA
Green Tube
Heparin
Dark Blue Tube
Heparin
Gray Tube
Sodium Flouride/Potassium Oxalate
Yellow Tube
Acid Citrate Dextrose
Light Blue Tube
Sodium Citrate
Acanthocyte
Erythrocyte that has irregular shaped margins or projections from the cell wall; Spur Cells
Leptocyte
Erythrocyte that is misshaped or folded over due to an increased membrane with decreased volume; RBC that is wafer-thin, generally large in diameter, with Hgb concentrated in a thin rim at the periphery
Horse
Animal with Rouleaux normally seen in their blood
Heinz Bodies
Erythrocyte common in cats and has a round area of denatured hemoglobin attached to the cell membrane
3-4 Days
How long does it take to stimulate the erythrocytes if there is an increased demand for them
Grey Top Tube
Used for glucose testing
Green Top Tube
Used for blood pH and ammonia testing
Light Blue Top Tube
Used for Coagulation Studies, Pt & PTT
Purple Top Tube
Used for General Hematology
Yellow Top Tube
Used for Transfusions
Tubes used for blood chemistries
Green and Tiger Top
Hemolysis
RBC Destruction
Fibrinogen
Protein that makes up the matrix to forma clot
g/dl
Units for Hgb measurement
Liver
Where plasma proteins are mainly produced
Erythroid
Myeloid line RBCs come from
Albumin
Protein that makes up 35-50% of the total protein
Icteric
If the plasma appears yellow in color, we denote this finding as
10-15 g/dl
Normal Hgb range for a Cat
5.4-7.6 g/dl
Normal TP range for a Dog
Buffy Coat
WBCs and Platelets that sit between RBCs and Plasma/Serum in a hematocrit
6,250,000/ul
Normal erythrocyte range for Dogs and Cats
Thrombocytes
Produced by the megakaryocytic line
PCV
Packed Cell Volume
TP
Total Protein
Hgb Estimation Equation
1/3 of PCV = __g/dl
5.7-7.6 g/dl
Normal TP value for Cat
Macrocytosis
Large Cells
Hypochromasia
Increased central pallor, lack of staining
Polychromatophil
Large cell with blue cytoplasm; reticulocyte when stained with NMB
Spherocyte
Small, round cells lacking central pallor; smaller than normal RBC but have same volume
Anisocytosis
Variation in RBC size
Poikilocytosis
Variation in RBC shape
Basophilic Stippling
Small, blue-staining granules
Nucleated RBC
Normal in birds and reptiles (metarubri)
Echinocyte
Burr Cells; Spiculated RBCs; projections may be sharp or blunt, are usually numerous and tend to be evenly spaced around the circumference (usually uniform in size)
Normochromic
Normal amount of hemoglobin in RBC
Howell-Jolly Bodies
Micronuclei; Small fragments of non-functional nucleus which were not extruded as the RBC left the bone marrow; Often noted in intravascular destruction of RBCs
Microcytosis
Increase in the number of smaller than normal RBCs
Polychromasia
Many colors; "Purpley-Bluey"; Polychromatophil
Hyperchromatophilic
No central pallor; smaller than normal RBCs
Rouleaux
RBCs stacked on top of each other and form a column/row. Looks like a stack of coins.
Agglutination
A large clump of RBCs
Crenation
Soft, little spikes on the outside of RBC; looks like wrinkles; blood left in EDTA too long
Drepanocytes
"Sickle-Cells"; Sickle-shaped cells
Keratocytes
Helmet Cells; RBCs with a blister-like vesicle, which may rupture, leaving a "bite-shaped" defect in the cell outline or one or two horn-like projections on the same side of the cell
Anulocytes
Bowl-shaped cells; Perfectly round and have a tiny amount of Hgb around the outside edge (rim of the bowl)
Codocyte
Target Cells; Have a "lump" of hemoglobinized cytoplasm within the area of normal central pallor causing them to resemble a "bullseye" target
Stomatocyte
RBC with an elongated (mouth-like) area of central pallor
Dacrocyte
Tear Cell; Tear drop shaped cells; some have central pallor and some do not
Schistocyte
RBC fragments; A wide variety of forms may be observed
Reticulocyte/Polychromatophil
Larger than mature RBC with granules (organelles) in the cell; NMB
Ghost Cells
RBC that has had all of its cytoplasmic contents removed by cell lysis so that only its outer cytoplasmic membrane remains
Stain Precipitate
Appears as purple granules or purple dots all over the slide