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pGLO

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plasmid
A small circular piece of DNA
transformation
altering or inserting a gene to change an organism;s traits
E. coli
What type of bacteria did we use?
4 agar plates
How many agar plates were used?
araC, GFP, bla, ori
What are the four genes present in the pGLO plasmid?
agar plates with LB broth
What type of medium did we grow E. coli on?
insert pGLO into E.coli to make it glow
What was the transformation we did in the experiment?
araC gene
protein that breaks down arabinose
GFP gene
green fluorescent protein
green fluorescent protein
WHat does GFP stand for?
bla gene
Beta lactamase protein that causes resistance to ampicillin
make the E.coli DNA more accepting of pGLO plasmid
Adding calcium chloride did what to the E.coli?
heat shock
rapid changes in temperature that cause the pores in bacteria membrane to better accept plasmid
ice, water bath, ice
What are the steps of heat shock?
arabinose
on and off switch for GFP gene
expressed
If arabinose is present, GFP is expressed or not expressed?
+pGLO, LB/amo/ara
WHich agar plate glowed?
-pGLO, LB, amp
Which agar plate had no E.coli growing?
-pGLO, LB
What was the control agar plate to make sure bacteria was growing?
-pGLO, LB, amp
What was the control group to make sure ampicillin was present?
jellyfish Aequorea victoria
What is the real-life source of the GFP gene
bacteria but no glow
WHat do you expect to occur in the agar plate with +pGLO, LB, amp?
bacteria and glow
WHat do you expect to occur in the agar plate with +pGLO, LB, amp, ara?
nothing
WHat do you expect to occur in the agar plate with -pGLO, LB, amp?
bacteria, no glow
WHat do you expect to occur in the agar plate with -pGLO, LB?