Only $35.99/year

Terms in this set (36)

A) Breaks hydrogen bonds, unwinding DNA double helix.
B) Synthesizes RNA primers on leading and lagging strands.
C) Replaces RNA primers with DNA nucleotides.
D) Catalyzes phosphodiester bond formation, joining DNA fragments.
E) lagging strand
F) Leading Strand
G) Relaxes supercoiled DNA.
H) Coats single-stranded DNA, preventing duplex formation.
I) Synthesizes DNA 5' to 3' on leading and lagging strands.

uring replication, DNA synthesis occurs in the 5′ to 3′ direction along both template strands.
On one template strand, synthesis proceeds continuously toward the replication fork, generating the leading strand.
On the other template strand, DNA is synthesized away from the replication fork in segments called Okazaki fragments, generating the lagging strand.
Several proteins are involved in DNA replication, including the following:
Helicase breaks the hydrogen bonds between the parental DNA strands and unwinds the double helix.
Single-stranded binding proteins bind to the single strands of DNA, preventing them from reannealing and allowing synthesis to occur on both strands.
DNA polymerase III synthesizes the new strands, but it requires an existing 3′ hydroxyl (—OH) group to add nucleotides.
Primase creates short RNA primers, initiating DNA synthesis on both template strands.
DNA polymerase I removes the RNA primers and replaces them with DNA nucleotides.
On the lagging strand, DNA ligase joins Okazaki fragments by forming phosphodiester bonds between them, thus completing DNA replication.