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Lab 33, 34, 40, 41. 47 Agglutination, Enteropluri, Phenol red, Triple sugar, Bacteria unknowns

Terms in this set (21)

Agglutination reactions
happen when antibodies bind to particular antigens or cells such as bacteria. The interactions are either visible or clumping of cells.
Passive agglutination
Passive agglutination assays utilize beads of latex or other materials to make the reaction visible. Either antigens or antibodies can attach to the beads. When antigen has been attached to the beads, they will agglutinate if the appropriate antibody is in the patient's serum.
C-Reactive protein (CRP)
is a serum protein that is elevated in inflammatory conditions such as bacterial infections and rheumatoid arthritis.
If the specimen is negative for CRP
no agglutination ( a uniformly turbid suspension) will result.
The latex agglutination slide test uses
Consists of latex beads coated with antibodies against human CRP.
Identification of unknown bacteria (5 steps)
1. Obtain an unknown substance (each tube has two different bacteria)
2. Make isolation
3. Add isolated colony to nutrient agar
4. Gram stain
5. Do biochemical tests to identify them
No CRP or little CRP is better for the patient or not?
Low CRP is good for the patient
The phenol red broth contains what? (5 of them)
-pH indicator phenol red
-Sugar of interest
-Salts
-Petone
-Durham tube
Durham tube
gas that are released from the cells accumulate. This is evidence of fermentation.
Phenol red turns the yellow
Acidic condition occurred. This means that acid is released into the medium and pH decreases red turns yellow. It means that acid production and indirectly indicated that fermentation has occurred.
Phenol red turns a bright fuchsia red color
Alkaline condition occurred, the phenol red turns bright fuchsia red color. Ammonia increases the pH of the medium. This organism lacks a transmembrane sugar transporter, the sugar cannot be metabolized. Organism usually metabolizes the peptone.
Reversion
Yellow tube at 24 hours can revert to a red or fuchsia or yellow with red top at 48 hours of incubation. Bacteria will eat the carbohydrates and when that becomes exhausted the organism will catabolize (break down) other available nutrients in the environment.. maybe peptone.
TSI tests for three sugars
Lactose
Sucrose
Glucose
Which sugar is a disaccharide and which sugar is a monosaccharide?
Lactose- disaccharide
Sucrose- disaccharide
Glucose - monosaccharide
TSI means what
Triple-Sugar iron
Hydrogen sulfide (H2S)
Some organism utilize sulfur compounds as terminal electron acceptors under oxygen-limiting conditions. Hydrogen sulfide (H2S) is one of those.
What is the problem with Hydrogen sulfide (H2S)?
Hydrogen sulfide (H2S) is a invisible gas.
How can you see Hydrogen sulfide (H2S)?
an iron salt is produced in the medium to detect it. The iron salt reacts with hydrogen sulfide to produce iron sulfide, which is visible. This iron sulfide is a black precipitate in the medium. H2S positive if we see the black in medium
A Multitest System
is a single-piece unit that may run from 10 to over 100 tests at the same time.
The EnteroPluri-Test can find out how many results?
15 different results. 12 compartments
What does the layer of wax generate?
The layers have wax to generate anaerobic conditions.
The layers have wax to generate anaerobic conditions.
Sets found in the same folder