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Biochemistry of RBC

Terms in this set (24)

Cytoskeletal proteins that give RBC's their flexibility
Spectrin, Ankyrin, Band 4.1, and band 4.2
What is hereditary spherocytosis?
Cytoskeletal defect of RBCs that leads to an intrinsic hemolytic anemia
Population that is most commonly affected by hereditary spherocytosis?
Northern european ancestry (1:2000)
Pattern of inheritance for hereditary spherocytosis
75% are AD
Most CO2 in the blood is transported as...
Bicarbonate (HCO3-)
Hemoglobin is a paralog of _____________
myoglobin
Structure of Adult hemoglobin
Quaternary protein structure
Heterotetramer of:
2 alpha chains, 2 beta chains
Structure of Fetal Hemoglobin
Quaternary protein structure
Heterotetramer of:
2 alpha chains, 2 gamma chains
The main functional difference between myoglobin and hemoglobin
Different affinity for oxygen at low partial pressures

Myoglobin's saturation curve is hyperbolic
Hemoglobin's saturation curve is sigmoidal
What property accounts for the functional difference of myoglobin and hemoglobin?
Allosterism of hemoglobin, affinity for oxygen changes after oxygen is bound
pH effect on O2 binding to Hb is known as ______________________
The Bohr Effect
Decrease in pH _________________ O2 release from hemoglobin
Enhances
Increase in pH _______________ O2 release from hemoglobin
Decreases
Decreases in pH stabilizes the _________ state of hemoglobin
T (tense)

lowers affinity for O2
Increases in pH stabilizes the ________ state of hemoglobin
R (Relaxed)

Increases affinity for O2
Effect of 2,3-BPG on hemoglobin's affinity for O2 and its effect on the saturation curve.
Lowers O2 affinity

Higher 2,3-BPG facilitates unloading of O2 at the tissue level

Shifts saturation curve to the right
2,3-BPG stabilizes the ______ state of hemoglobin
T (tense)
2,3-BPG binds where on hemoglobin?
Binds to deoxy hemoglobin in the cleft between the two beta chains
Most significant buffer system in the blood
Bicarbonate buffer system
Write out the chemical equation that depicts the bicarbonate buffer system of the blood, including enzymes for each step.
Saturation curve of fetal hemoglobin compared to adult hemoglobin
Shifted to the left vs. the HbA curve
Main source of energy for RBCs
Glycolysis/Anaerobic respiration
Pyruvate kinase deficiency can cause what pertaining to the blood.
Hemolytic anemia
Define cooperativity as it pertains to RBCs
Binding of one oxygen molecule affects the binding of subsequent oxygen molecules.

Binding of one oxygen decreases affinity
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