Upgrade to remove ads
BI220 (Controlling Microbial Growth)
Terms in this set (36)
True or False: In modern surgery, we use rigorous cleaning procedures to maintain sepsis
What particularly robust bacterial form must be killed for a technique to be considered true sterilization?
What is the difference between disinfection and antisepsis?
- Disinfection: The treatment of inanimate objects
- Antisepsis: The removal of microbes on living tissues
Which term means to wipe off bacteria?
If a public safety technician finds bacteria on a fork, would they conclude that the fork was not properly sanitized?
- It depends
- Question to ask oneself: Is there an acceptable number of colonies present on the fork?
What is the maximum temperature of water vapor under normal conditions?
- 100 degrees C (212 degrees F)
How does heat kill microbes?
- It denatures proteins
Why is it desirable for instruments that are being autoclaved to be wrapped in pouches that are permeable?
- Because the steam is actually doing the killing, not just the air
How do autoclaves achieve internal temperatures that exceed the normal maximum temperature of steam?
What is the benefit of the high-temperature moist heat that autoclaves are capable of producing?
- It is capable of killing endosperms and takes less time to kill vegetative cells
What was Louis Pasteur trying to preserve when he invented pasteurization?
- Beer and wine
How does pasteurization differ from other methods of moist heat?
- Can be maintained at a lower temperature
What disease helped encourage the adoption of pasteurization of milk?
- Tuberculosis (TB)
Why does the temperature of dry heat need to exceed the temperatures used for moist heat sterilization?
What are the modes of cell death in dry heat sterilization?
- Protein denaturation, burning,
desiccation (Drying out)
When is vacuum filtration desired over heat sterilization?
- When a solution needing to be sterilized is sensitive to heat
What type of UV light is the best for killing microbes and why?
- UVC because the absorption spectrum of DNA is closely matched to that of UVC
How does UV light act on microbial cells to cause their death?
- Thymine dimers causes mutations
Explain how a robot is helping some hospital decrease the occurrence of C. difficile infections?
- Through the use of UV light when cleaning rooms
In what century were disinfectants first used to prepare instruments for surgery?
What concentration of isopropyl alcohol is the most effective for killing bacteria?
What common disinfectants is considered halogens?
- Chlorine and iodine
Why is hypochlorous acid particularly good at killing microbes?
- It has a neutral charge, thus, it can penetrate cells
What is the best supported idea about the antimicrobial activity of iodine?
- It connects two amino acids, resulting in a change in the shape of the protein
How does hydrogen peroxide use oxygen to kill microbes?
- Strips hydrogen from other important molecules
What cell structure is dissolved by isopropyl alcohol?
- Plasma membrane
Why is 70% alcohol more effective in killing microbes than solutions that contain higher concentrations of alcohol?
- It does not coagulate the proteins of the outer covering
Who pioneered the use of disinfectants in the 1800's?
- Joseph Lister
Why was phenol later abandoned and replaced by other antiseptics?
- It is costly to skin
How does Lysol kill bacteria?
- Chops off fat molecules
Explain the difference between bactericidal and bacteriostatic antibiotics
- Bactericidal: Kills
- Bacteriostatic: Slows or ceases growth
What processes might be affected by a bacteriostatic antibiotic?
- DNA production
- Protein synthesis
- The function of enzymes
Are penicillins bactericidal or bacteriostatic?
What is the difference between a broad-spectrum and a narrow-spectrum antibiotic?
- Narrow: Effects a specific pathogen
Broad: Kills everything
Why would we choose a narrow-spectrum antibiotic instead of a broad-spectrum?
- Good bacteria is being killed with the bad bacteria
When are broad-spectrum used?
- When there is an absence of time
YOU MIGHT ALSO LIKE...
Microbiology Ch. 7
Microbiology Ch. 7
Microbiology Ch. 9 LAMC
OTHER SETS BY THIS CREATOR
"Need to Know" Laboratory Values
NR 365 (Chapter 42: Drugs for Bowel Disorders and…
NR 365 Chapter 41 (Drugs for Peptic Ulcer Disease)
NR365 Chapter 34: Drugs for Immune System Modulati…