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Microbiology LAB--- Test Review
Terms in this set (52)
Gram Positive cells
Gram Negative cells
Clustered circular bacteria (grape-like)
2 circular shaped bacteria in a row
4 circular shaped bacteria (2 on the bottom and 2 on the top)
2 rod shaped bacteria in a row
rod shaped bacteria in a chain
not circular or rod but in between (egg-shaped) (Oval-shaped)
"C" or "S" Shaped bacteria
Curvy-Shaped bacteria with no more than 3 curves on one side
Curvy-shaped bacteria with MORE than 3 curves on each side.
The scanning objective has a magnification of ________.
The Low Power objective has a magnification of ________.
The High Power Objective has a magnification of ________.
The Oil Immersion objective has a magnification of ________.
The oculars have a _______ magnification.
Which objective is the longest?
100x (oil immersion objective)
Which objective do you use the oil immersion with?
100 x (oil immersions objective)
Which objective is the shortest and safest distance from the slide?
What is the total magnification if you are looking at a specimen on the low power objective and you are using an ocular with a magnification of 10x?
What is the total magnification if you are looking at a specimen on the scanning objective and you are using an ocular with a magnification of 10x?
What is the total magnification if you are looking at a specimen on the high power objective and you are using an ocular with a magnification of 10x?
What is the total magnification if you are looking at a specimen on the oil immersion objective and you are using an ocular with a magnification of 10x?
How do you carry the microscope?
One hand on the arm and one hand under the base
What part of the microscope moves the objectives?
Eyepieces are also known as the _________________.
What knobs moves the stage up and down?
Course and fine adjustment knobs
What knobs moves the stage side to side?
What do you clean the microscope lens' with?
Lens cleaner and kim wipes
When you begin to first look at a specimen, you should start with which objective?
4x (Scanning) or 10x (low power)
After performing the gram stain, gram positive cells will retain the color_____________.
After performing the gram stain, gram negative cells will retain the color _________________.
What are the steps in a wet mount?
1. start with a clean slide
2. place yeast cells on slide
3. place safranin dye on the yeast cells
4. place cover slip on the yeast cells and safranin dye.
5. place a paper towel beside the cover slip on each side to draw out the excessive water and dye.
6. view on 10x and then on 40x
What are the steps in a simple stain?
1. start with a heat fixed smear prep.
2. apply crystal violet to the bacteria.
3. wait for 60 seconds.
4. wash off till it runs clear.
5. blot it with bibulous paper.
6. view on 10x, 40x, and then 100x with oil.
What are the steps in a gram stain?
1. start with a heat fixed smear prep
2. Apply crystal violet dye, wait 60 seconds, then wash off till it runs clear.
3. Apply iodine, wait 60 seconds, then wash off till it runs clear.
4. dip slide in alcohol, dip 4 times, wash off till it runs clear.
5. Apply safranin dye, wait 60 seconds, then wash off till it runs clear.
6. blot it with bibulous paper.
6. view on 10x, 40x, and then 100x (with oil only on the 100x objective).
What is the purpose of performing a gram stain?
To distinguish between a gram positive cell wall and a gram negative cell wall.
What is the purpose of performing simple stain?
To highlight the cell features so you can identify the cell's shape.
What is the purpose of performing a smear prep?
The smear prep adheres the bacteria to the slide so it is not washed off during the staining process.
Yeast-like bacteria have the cell shape of_______?
Round bacteria are called __________________.
coccus or cocci
Rod bacteria are called ____________________.
Curvy bacteria that have the 'C" or "S" shape are called______?
Curvy bacteria that have 3 curves on at least one side is called_____.
Curvy bacteria that have more than 3 curves (generally a lot of curves) on both sides is called ____________________.
Round bacteria in a line (chain) are called_____.
Round bacteria that are clustered are called_______.
Two round bacteria are called ______.
Two rod bacteria are called ______.
4 round (2 on the top and 2 on the bottom) bacteria are called_____.
Rod bacteria in a chain (line) are called______________.
What are the steps in performing a smear prep?
1. start with a clean slide
2. draw a circle in the middle of your slide
3. Sterilize your loop, place a drop of water on your loop and transfer the water to the middle of your circle on your slide.
4. Sterilize your loop, wait up to 60 seconds, grab the bacteria from you petri dish (or tube) and transfer it to the water on your slide.
5. mix the bacteria with the water and then spread it (thin it out) out within the circle.
6. let it air dry
7. heat fix it.
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