Bel Rea Hematology Exam 1
Terms in this set (56)
What is the #1 mistake in Hematology?
Not gently and thoroughly mixing blood
The fluid portion of anti-coagulated while blood
The fluid portion of clotted/coagulated whole blood
PCV (Packed Cell Volume)
The percent of a quantity of blood which is made up of the red blood cells.
What makes up the buffy coat?
WBC and platelets
At what time and speed should crit tubes spin?
6000 RPM for 5 min
What are the terms of quantification?
mild, moderate, marked
What are the two normal colors that plasma can be?
Clear or straw
What is the term for red plasma?
What is the term for yellow plasma?
What is the term for white plasma?
What are the causes of hemolytic plasma?
shaking, too small of a needle with too much negative back pressure, IV himolysis (IVH)
What are the causes of icteric plasma?
biliary stasis, carotenes, ev hemolysis (increased EVH)
What are the causes of lipemic plasma?
postprandial, hypothyroid, DM
What is in Jar 1 of Dif Quick stain, and what is the procedure?
Fixative - 5, one second dips
What is in Jar 2 of Dif Quick stain, and what is the procedure?
Eosinophilic - 5, one second dips
What is in Jar 3 of Dif Quick stain, and what is the procedure?
Basophilic - 7-10, one second dips
What is in Jar 1 of Wright's stain, and what is the procedure?
Wright's stain - place blood film in jar 1 for 1-3 min.
What is in Jar 2 of Wright's stain, and what is the procedure?
Buffer w/ 1 pipette of Wright's stain - place blood film in jar 2 for 1 min.
What is in Jar 3 of Wright's stain, and what is the procedure?
Distilled water - 7-10, one second dips
Chain of RBCs. Normal in healthy equines. Common in dehydrated patients.
RBCs clumping together and overlapping due to antigen antibody binding. Not normal in any species for any reason.
What is evaluated on 10x in the feathered edge of the blood film?
1.) WBC distribution
2.) Platelet clumping
3.) Abnormal cells
What is evaluated on 10x in the monolayer of the blood film?
1.) Adequate size?
2.) WBC distribution
What is evaluated on 10x in the body of the blood film?
RBC associations (rouleaux, agglutination)
What is evaluated on 40x in the feathered edge of the blood film?
What is evaluated on 40x in the monolayer of the blood film?
1.) WBC differential
2.) Begin morphology assessment
What is evaluated on 40x in the body of the blood film?
What is evaluated on 100x in the feathered edge of the blood film?
What is evaluated on 100x in the monolayer of the blood film?
1.) RBC morphology
2.) WBC morphology
3.) Platelet assessment
What is evaluated on 100x in the body of the blood film?
A membrane bound package of substances
A membrane bound package lacking substance
Granules in cytoplasm. Elongated, dark, dense, segmented nucleus. Dark chromatin.
The pattern/organization of genetic material within the cell. The nuclear characteristics.
Neutral staining cytoplasmic granules. Segmented nucleus. Clumped, ropey chromatin.
Red-orange staining cytoplasmic granules. Segmented nucleus.
Grey-blue to purple staining cytoplasmic granules. Segmented nucleus. Very rare!
ameboid nucleus, lacy chromatin pattern, blue-grey cytoplasm +/- vacuoles.
High nucleus to cytoplasm ratio. Clumped/condensed chromatin. Small cap of cytoplasm.
List the normal RBC sizes for small and large animals from smallest to largest
Sheep (4.5 um)
Cattle (5.5 um)
Horse (5.7 um)
Cat (5.8 um)
Swine (6 um)
Dog (7 um)
Variation in size of RBC
Unusually large mature RBCs (very rare)
Unusually small mature RBC
Term used to describe overall appearance of the blood film.
Crenated cells. Small, even projections over the entire cell surface.
Irregular spicules that are uneven in size and distribution.
Have one or two long spikes coming off the cell. Looks like a helmet or Elmer Fudd Hat.
RBC with more surface area than contents
Variation of a leptocyte that looks like a bulls eye target.
Small, round, evenly staining RBC. More contents than surface area.
RBC has a pale colored mouth area in the center of the cell.
"Punched out cell". Hole in the middle
"Blister cell". Common in conjunction with Heinz bodies. Due to oxidization of the red cell membrane.
Literally an empty RBC membrane. Usually an indication of IVH.
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THIS SET IS OFTEN IN FOLDERS WITH...
Bel-Rea Hematology: Quiz 4 (Fall 2016)
Bel-Rea Hematology: Quiz 5 (Fall 2016)
Bel-Rea Hematology: Quiz 7 (Fall 2016)