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What is the purpose of the mordant, Iodine?
Keeps the Crystal Violet from being removed from the Gram-positive bacteria
What does the Decolorizing Solution, Ethanol, do to Gram-negative bacteria? Why?
Washes out the purple complex. Cell walls are too thin
What does the Decolorizing Solution, Ethanol, do to Gram-positive bacteria?
Nothing, because of the thick cell walls
What happens to the cell wall if they are old or are stressed by heat or cold?
The cell walls become thin
What are the two groups of bacteria and their colors?
Gram-positive and Gram-negative bacteria; purple and pink, respectively
The bacterial cell wall is composed of what? Where is it located?
Peptidoglycan; on top of the plasma membrane
Gram-negative cell walls contain two...? Which are? What's in the middle?
Membranes; inner and outer; peptidoglycan
What does CFU stand for and what does it mean?
Colony Forming Units; the number of colonies grown during incubation
How much of the broth sample do we obtain and where do we put it?
We obtain 1.0 mL; put it in the 10^-1 test tube
What do you do after diluting the test tubes? How much do we obtain? 10^-4 turns into?
Dilute again into petri dish; 0.1 mL; 10^-5
What are the steps to using the spreading rod?
Dip in alcohol, pass through burner; let cool; spread specimen
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