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Microbiology Lab Final
Terms in this set (37)
There are two circle pieces under the microscope stage, what are they
Condenser on top, Iris diaphragm on bottom
what is the goal of a streak plate?
obtaining isolated colonies
staining technique that uses only one die to observe bacterial shape
uses 2 or more dyes to distinguish between different types of bacteria based on their physical and chemical properties
what color do gram- and gram+ stain?
gram - are pink, gram + are purple
order of gram stain procedure
a non-selective media that contains ingredients that promote bacterial growth
media that contains special ingredients that can help distinguish different types of bacteria and/or grow fastidious bacteria
a type of enriched agar that contains dyes or toxic substances which will inhibit the growth of certain bacteria while promoting the growth of other bacteria
a type of enriched media which differentiates or distinguishes between different types of bacteria based on differences in appearance of growth or color change
what type of media is MAC and how does it differentiate between bacteria
MacConkey's is a differential agar that can distinguish between bacteria that can/cannot ferment lactose. Bacteria colonies that can ferment turn PINK and bacteria that can not remain COLORLESS.
what type of media is MSA and how does it differentiate between bacteria
Mannitol Salt Agar is a differential agar that can distinguish between bacteria that can/cannot ferment mannitol. Bacteria colonies that can ferment turn the AGAR YELLOW and bacteria that can not, AGAR remains PINK.
test determines if certain gram-negative bacteria metabolize glucose by fermentation (without oxygen) or aerobic respiration (with oxygen)
The O/F Test contains two tubes. The media in both tubes is ________ before the test is conducted
During the O/F test, if the bacteria can metabolize glucose, the media will turn...
are fungi eukaryotic or prokaryotic?
two main groups of fungi
molds (multicellular) and yeasts (unicellular)
what type of media is SAB and how does it work?
Sabouraud Agar is a selective agar that contains sugar and antibiotics and is very acidic, which prevents bacteria from growing while allowing both mold and yeast to go
eukaryotic, microscopic, single-celled organisms that can be free-living or parasite
the passing of a pathogen causing disease from an infected host to a particular individual or group
what are the 3 parasitic protozoan modes of transmission?
1. fecal/oral route
2. Sexually transmitted
3. Vector (living organism that can transmit infectious diseases between humans or from animals to humans)
a virus that specifically infects bacteria
5 phases of the lytic cycle
the destruction of all forms of microbial life, with particular attention to bacterial spores
the killing of many, but not all microorganisms. It is a process of reduction of number of contaminating organisms to a level that cannot cause infect
a biological or chemical agent that stops bacteria from reproducing, while not necessarily killing the
a biological or chemical agent that is capable of completely killing bacteria
a dormant, tough, non-reproductive structure produced by a small number of bacteria that will preserve the cell's genetic material in times of extreme stre
Endospore stain: what is primary stain and counterstain?
primary: malachite green
endospores will stain....
A method used to determine the sensitivity of bacteria to specific antibiotic
How do we measure resistant of antibiotics for the Kirby-Bauer Test
zone of inhibition
A collection or community of microbes
the native, non-disease causing microbial organisms that reside in and on healthy individual
microbial organisms that are picked up from the environment and may cause disease
Gram stain of staphylococcus? shape? grow on TSA and BAP? grow on MSA?
Gram Positive Cocci. ALL will grow on TSA and BAP. ALL will grow on MSA; only some will ferment mannitol.
Gram stain of streptococcus? shape? grow on TSA and BAP? grow on MSA?
Gram Positive Cocci. ALL will grow on TSA and BAP. Most will NOT grow on MSA
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