Terms in this set (22)
the direct manipulation of genes for practical purposes
the use of technology to determine the exact sequence of an organisms genome
the use of a vector to make multiple copies of a particular gene
small circular DNA molecules that are replicated separately from the majority of the bacterial DNA
a molecule containing DNA from two different sources
a DNA molecule that can carry foreign DNA into a host cell and replicate there
enzymes that cut viral DNA at specific points within the restriction site
a particular short DNA sequencing recognized by a restriction enzyme
single stranded overhaning regions that result from DNA being cut by restriction enzymes
an enzyme that is used to connect DNA fragments together
a process through which DNA stands are separated by charge and weight as they move through a porous gel; can also be used to separate proteins and RNA fragments
Polymerase chain reaction
a process by which many copies of a desired gene can be created quickly using a thermal cyler machine
a cloning vector that contains a highly active bacterial promoter just upstream of a restriction site that allows the inserted gene to be expressed readily
RNA interference (RNAi)
miRNA bind to certain mRNA fragments and keep them from being expressed
a relatively unspecialized cell that can give rise to other cells and can reproduce itself indefinitely
an animal that contains genes from another animal
Short tandem repeats
tandemly repeated units of 2-5 bases in specific regions of the genome the number of which vary greatly from person to person
a testable explanation a supposition or proposed explanation made on the basis of limited evidence as a starting point for further investigation.
a group of subjects that are not given the treatment being tested in order to serve as a benchmark for the tested group
an explanation of an aspect of the natural world that can be repeatedly tested, in accordance with the scientific method, using a predefined protocol of observation and experiment
a variable (often denoted by x ) whose variation does not depend on that of another.
a variable (often denoted by y ) whose value depends on that of another.
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