DNA Replication

Suggest the consequences for daughter cells if DNA replication did not take place prior to mitosis
If DNA didn't replicate the daughter cells wouldn't be able to have a complete strand
List four requirements requirements for DNA replication
Double helix unwinds, individual free nucleotides, DNA polymerase, semi-conservative
What is meant by the semi-conservative model
When the double strand separates, each parental DNA strand acts as a template to form a complimentary strand so that two identical daughter DNA molecules are formed
State the two enzymes that are involved in DNA replication and describe their functions
Helicase - unwinds a portion of the helix
Polymerase - pairs up bases
State the direction that DNA replication occurs in
5' to 3'
Explain the importance of complimentary base pairing in conserving the base sequence during DNA replication
DNA replication is semi-conservative. This complementary base pairing allows the two DNA molecules to be identical because they have the same base sequence. The new strands formed are complementary to their template strands but also identical to the other template
Describe what complimentary means with respect to DNA replication
Contains the template information for the creation of a new copy of the other strand
State the enzymes involved in DNA replication
Helicase, Primase, DNA Polymerase III, DNA Polymerase I, DNA Ligase
Sequence of events in DNA replication on the lagging strand
1. Helicase causes the helix to uncoil and the bases become exposed
2. RNA primase adds a short RNA primer to the 3' to 5' strand
3. Okazaki fragments are formed
4. DNA polymerase III adds nucleoside triphosphates to the strand working in a 5' to 3' direction away from the replication fork
5. DNA polymerase I replaces RNA primers with DNA
6. DNA ligase joins Okazaki fragments together