DNA strands can be clipped crosswise at selected positions by using enzymes called
Sequences of DNA that are identical when read from 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are
Larger fragments move slowly and remain closer to the wells; DNA has an overall negative charge and moves to the positive pole; DNA fragments are stained to see them; An electric current through the gel causes DNA fragments to migrate.
Analysis of DNA fragments in gel electrophoresis involves
Recombinant stains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:
Can be engineered to become factories for manufacturing proteins; Are often obtained from germ line engineering; Will pass the genes on to their offspring; Commonly includes mice
When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called
This is often used in forensic science to distinquish one sequence of DNA from another by comparing the sequence of the strands at specific loci:
Has a slow growth rate
All of the following are desirable features in a microbial cloning host except it
Human growth hormone, Factor VIII, insulin, and tissue plasminogen activator
Which of the following drugs is produced by genetic engineering and approved for human use?
Which of the following is less subject to degradation than in chromosomal DNA and is used as an evolutionary time clock?
Forensics, evolutionary studies, gene mapping,and medicine
The polymerase chain reaction (PCR) has been used in all of the following fields
It cannot be used on specimens older than 5 years
All of the following are correct about DNA fingerprinting except
After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.
gene therapy False
An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.
Transformation and transfection are methods used to introduce DNA into host cells.
normal gene False
The process of introducing a needed, normal gene, into human cells is called DNA mapping.
Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.