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31 terms

Chapter 10 quiz

Chapter 10 quiz was changed a little so it could work on quizlet
The use of an organism's biochemical processes to create a product is
Genetic engineering
The various techniques by which scientists manipulate DNA in the lab are called
Restriction endonucleases
DNA strands can be clipped crosswise at selected positions by using enzymes called
Restriction endonucleases
EcoRI and HINDIII are
Sequences of DNA that are identical when read from 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are
Larger fragments move slowly and remain closer to the wells; DNA has an overall negative charge and moves to the positive pole; DNA fragments are stained to see them; An electric current through the gel causes DNA fragments to migrate.
Analysis of DNA fragments in gel electrophoresis involves
Enzymes, fluorescent dyes, and radioisotopes
Gene probes can be labeled for detection with
Included Taq polymerases and Vent Polymerases
DNA polymerases used in PCR
The western Blot technique detects
Synthetic DNA oligonucleotides
The primers in PCR are
Cloning host takes up a plasmid
Which step involves transformation?
Pseudomonas fluorescens
Recombinant stains of this organism are released to colonize plant roots to produce an insecticide to destroy invading insects:
Can be engineered to become factories for manufacturing proteins; Are often obtained from germ line engineering; Will pass the genes on to their offspring; Commonly includes mice
Transgenic animals
Gene therapy
When patient tissues are transfected with viruses carrying a needed, normal human gene, the technique is called
DNA fingerprinting
This is often used in forensic science to distinquish one sequence of DNA from another by comparing the sequence of the strands at specific loci:
Has a slow growth rate
All of the following are desirable features in a microbial cloning host except it
Human growth hormone, Factor VIII, insulin, and tissue plasminogen activator
Which of the following drugs is produced by genetic engineering and approved for human use?
Mitochondrial DNA
Which of the following is less subject to degradation than in chromosomal DNA and is used as an evolutionary time clock?
Forensics, evolutionary studies, gene mapping,and medicine
The polymerase chain reaction (PCR) has been used in all of the following fields
It cannot be used on specimens older than 5 years
All of the following are correct about DNA fingerprinting except
endonucleases True
Restriction endonucleases are obtained from various species of bacteria.
RNA True
Some genes in bacteria are repressed by antisense RNA that binds to them.
DNA True
When DNA is heated, the two strands will separate.
DNA False
After 3 replication cycles in PCR, there will be a total of 4 double-stranded DNA molecules.
gene therapy False
An example of gene therapy is the insertion of the gene for human growth hormone into E. coli cells.
cDNA True
Reverse transcription is used to make cDNA from an RNA template.
transfection True
Transformation and transfection are methods used to introduce DNA into host cells.
gene False
Sequencing a gene automatically identifies which gene it is.
normal gene False
The process of introducing a needed, normal gene, into human cells is called DNA mapping.
gene true
Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.
VNTR's and microsatellite polymorphisms are genetic markers seen in DNA fingerprinting