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Microbiology Lab Quiz Bacterial Transformation and Antibiotic Resistance

Terms in this set (21)

What occurs when a cell acquires and expresses a new piece of DNA
genetic transformation
When a new piece of DNA is acquired by an organism, is the new trait identifiable?
yes
In this lab, we used what bacterial cells
E. coli HB101
What plasmid DNA was used
pGLO
The pGLO plasmid contained gene for 2 traits - what were they?
production of green fluoroscent protein and resistance to ampicillin
In order for the traits to be expressed the genes must be connected to an ___
operon
What does the green fluorescent protein (GFP) in the pGLO plasmid use in order to be expressed?
arabinose operon
What switches on the arabinose operon
sugar arabinose
In the presence of ___, transformed cells will produce GFP and fluoresce under UV light
arabinose
The ampillicin reistance gene has it own operon ___
beta-lactamase enzyme
activity growing cells are added to the transformation solution and then the plasmid DNA is added - it is then incubated in ice - why
slow the fluid plasma membrane
after incubation in ice the solution is incubated at 42 degree celcius - why
increase the permeability of plasma membrane
The switching from ice to room temp facilitates what
entry of plasmid into cytoplasm
The switching from ice to room temperature method of transformation is called
heat-shock
What kind of broth was the transformated cells incubated in?
Luria-Bertani
What is used to test for antibiotic susceptibility
Kirby-Bauer
Kirby-Bauer is also called ___
disc diffusion assay
On the Kirby-Bauer test what kind of agar plate was used
Mueller-Hinton
The antibiotic concentration is ___ close to the dis and ___ with distance from the disc
highest decreases
What is the area where the organism does NOT grow on Kirby-Bauer
zone of inhibition
Results from the Kirby-Bauer will indicate whether the bacterium is __, ___ or ___ sensitive to the antibiotics tested
sensitive, resistance intermediately sensitive
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