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22 terms

Part 1

STUDY
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Acidic Stains
Are anionic, which means that the chromophore exhibits a negative charge and therfore has a strong affinity for the positive constituents of the cell.
Basic Stains
Are catioic, because the chromophore exhibits a positive charge and therefore has a strong affinity for the negative constituents of the cell.
Crystal Violet
This violet stain is used first and stains all cells purple
Grams Iodine
THis reagent serves as a mordant, a substance that increases the cells' affinity for a stain
95% Ethyl Alcohol
This reagent serves a dual function as a protien- dehydrating agent and as a lipid solvent
Safranin
This is the final reagent, used to stain red those cells that have been previously decolorized
Gram-Variable
This is, some cells will appear purple, whil others will appear pink
Vegetative Cells
Organisms that have the capacity to exist either as metabolically active
Spores
Highly resistant, metabolically inactive cell types
Endospores
Surrounded by impreviouslayers called spore coats
Spore
The endospore is released from the degenerating vegetative cell and becomes an independent cell
Malachite Green
Unlike most vegetative cell types that stain by common procedures, the spore, because of its imperveous coat, will not accept the primary stain easily
Water
Once the spore accepts the malachite green, it cannot be declorized by tap water, which removes only the excess primary stain
Capsules
Glycocalyx composed of repeating units of organic chemicals firmly attached to the cell surface
Carbol Fuchsin
A red phenolic stain that is soluble in the lipodial materials that constitue the major portion of the mycobacterial cell wall, does penetrate these bacteria and is rertained
Methylene Blue
This is used as the final reagent to stain previously decolorized cells
Brightfield Microscope
This instrument conains two lens systems for magnyfing specimens: The ocular lens in the eyepiece and the objective lens located in the nose piece
Principle
Microbiology is a science that studies living organisms that are too small to be seen with the naked eye
Stage
A fixed platform with an opening in the center allows the passage of light from an illuminating source below to the lens system above the stage
Illumination
The light source is positioned in the base of the instrument
Condensor
This component is found directly under the stage and contains two sets of the lens
Body Tube
Above the stage and attached to the arm