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Experiment #18: Normal Microbiota of the Throat and Skin
Terms in this set (33)
The population of microbes regularly found in the body is referred to as the
Members of the normal microbiota that are present for only a short time before disappearing are referred to as
Normal microbiota often inhibit pathogenic microbes from colonizing the host through a process called
Normal microbiota of the mouth:
Streptococcus (mutans, gordonii, and salivarius)
Found in approximately 5-15% of normal individuals as part of the normal throat microbiota.
Media used to differentiate between Streptococcus species:
Blood agar & Chocolate agar
*Blood plates containing these microbiota should be incubated in the presence of carbon dioxide
Members of the genus Neisseria are
fastidious organisms that require special media and conditions for growth.
Neisseria are typically cultivated on
Chocolate agar contains
lysed (boiled) red blood cells.
*Provides the nutrients and growth factors needed for the Neisseria genus
capnophiles, so chocolate plates containing Neisseria should be incubated in the presence of oxygen.
The pathogen Neisseria meningitidis is found in approximately
20-40% of young adults as a normal resident of the nasopharynx.
Neisseria are distinguished from other nasopharyngeal flora by
performing an oxidase test.
A purple/black color indicates the organism is
oxidase-positive and belongs to the genus Neisseria.
The oxidase test tests for
the presence of cytochrome c in the electron transport chain.
A species of the genus Corynebacterium that are non-pathogenic and are part of normal skin microbiota.
Diphtheroids are cultivated on
Mueller-Hinton Tellurite (MHT) plates.
Mueller-Hinton Tellurite (MHT) plate
If Diphtheroids are present,
they will produce gray to black colonies because the tellurite in the media is reduces intracellularly to tellurium, which appears as a gray precipitate.
Staphylococcus epidermis & aureus are
normal microbiota of the skin.
Staphylococcus aureus tends to be found
in moist environments such as the nostrils and armpits.
Staphylococcus epidermis is usually found
in drier areas of the skin.
Staphylococcus aureus is widely known as
a troublesome opportunistic pathogen.
Mannitol Salt Agar (MSA) selects for
both members of the Staphylococcus genus.
Mannitol Salt Agar (MSA)
Mannitol Salt Agar (MSA) differentiates
between Staphylococcus epidermis and aureus.
Staphylococcus aureus will
ferment mannitol to produce golden-yellow colony growth, whereas Staphylococcus epidermis will not.
Methicillin-resistant Staphylococcus aureus (MRSA):
An acidic medium (pH 5.5) which is used to cultivate acidophiles such as fungi
Sabouraud agar is selective for
Sabouraud agar promotes the growth of
Fungi (yeast & mold).
THIS SET IS OFTEN IN FOLDERS WITH...
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