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Genetics Test 2 Lecture 1 SI
Terms in this set (16)
1. Match each of the following experiments to their eventual conflusions:
1) Griffith's experiment
2) Avery's experiment
a) DNA is the genetic material of the cell
b) Something in virulent bacteria could transform virulent bacteria
c) DNA is the transforming material in bacteria
1- b; 2-c; 3-a
had virulent (smooth) and avirulent (rough) bacterial strains, when mice are injected with heat killed smooth bacteria they live, when injected with both living rough and heat killed smooth they die (heat killed smooth DNA transformed living rough cells)
3 different samples of heat killed smooth cells (one has no protein, one has no RNA, and one has no DNA), when rough cells were added to these samples, only the DNA-depleted sample did not transform
they radiolabeled phosphorus (DNA) and sulfur (proteins) in two different samples of T2 phages, then added to bacterial samples; in sulfur labeled samples the bacteria was not radioactive but phage skeletons were; in phosphorus labeled samples the bacteria was radioactive but phage skeletons were not
T/F: Because DNA has 2 hydroxyl groups, it is much more stable than RNA.
F - DNA is more stable, but because it only has 1 hydroxyl group
Which nucleotides are purines? Which are pyrimidines?
Purines = A and G, pyrimidines = C, T (DNA only), and U (RNA only)
Cytosine makes up 38% of the nucleotides in a sample of DNA from an organism. Approximately what percentage of the nucleotides in this sample will be thymine?
C (38%) + G (38%) = 76% ; 100% - 76% = 24% AT ; 24%/2 = 12% T
Name the three exceptions to Chargaff's Parity Rule.
Organellar genomes, single stranded viral genomes, and RNA viral genomes
Complete the complementary sequence: 3'ATTGCAATGCC5'
T/F: You cannot necessarily predict the next nucleotide in a strand of DNA.
What form of DNA is typical in our cells? What form of DNA is typically dehydrated and highly condensed?
B form; A form
What are the 3 differentiating factors of RNA?
Uses uracil instead of thymine, used ribose sugar instead of deoxyribose sugar, and is single stranded
Name the three different double-stranded structures that RNA/ssDNA can form.
Stem, hairpin, and cruciform
T/F: DNA can have a triple helix.
True - H-DNA, uses Hoogsteen base pairing
Which nucleotide(s) can be methylated in prokaryotes? Eukaryotes? What is the function of DNA methylation?
Cytosine and Adenine; Cytosine; silences genes
Which sequence is palindromic?
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