Using the 100X objective - scanning the monolayer for WBCs in a systematic manner. When a WBC is found press the appropriate button on the manual differential counter , 100 WBC are counted and the differential is reported in percentages; for example 55% segs, 4% bands, 26% lymphs, 9% monocytes, 5% eosinophils and 1% basophils. As a quality check the technician should always analyze the results to ensure that they equal 100%.