Biology 213 Exam 3 Chapter 5
Terms in this set (15)
Discovered inheritance functions through discrete units (gene); pea plants
Thomas Hunt Morgan
genes are carried on chromosomes
led to the discovery that gene loci were linked to specific chromosomal locations; this was discovered through the use of x-ray mutations in which identifiable regions were altered
Discovered Nucleic acids by identifying nuclein (later renamed nucleic acid), a protease insenstive (pepsin), acid-insoluble, alkali-soluble material from white blood cells. Nuclein was very large, acidic and rich in phosphorous
it was thought at the time (1930s) that proteins were in fact the genetic material because of their complexity
-showed that the base composition varied significantly from species to species
-it did not matter from which tissue or organ the DNA came from. Base composition was constant within the species.
-A=T and G=C, that is A and T were present in equimolar amounts as were G and C
showed that material could be transferred from a heat-killed virulent strain to a non-virulent strain, making the non-virulent strain virulent.
This process was called transformation.
Avery, MacLeod and McCarty
Determined that DNA was Griffith's "Transforming Factor."
Hershey and Chase
-using bacteriophage conclusively demonstrated that DNA contained the genetic information responsible for phage replication
-Labeled phage DNA with P32 and labeled phage proteins with S35, infected bacteria. Progeny phage contained P32
Watson and Crick
used to-scale molecular models based on the helical dimensions from the Franklin and Wilkins data and the data of Chargaff to propose a double helical structure where the helix was held together by the A-T and G-C base pairs
X-ray diffraction data suggested a helical structure used by Watson and Crick
Eric Davidson and Roy Britten
The Godfathers of Genomics and Bioinformatics
Roy Britten and Kohne
kinetics of renaturation of DNA
Polymerase Chain Reaction
contributed significantly to our understanding of the role of chromatin in regulating transcription