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Microbiology Lab Final

Terms in this set (94)

EMB agar use
Select for gram negative bacteria; differential for lactose fermenters
EMB agar media
Esosin Methylene blue agar
EMB agar positive result
growth = gram -; fast fermenters - dark green to black; slow fermenters - pink/maroon
EMB agar negative result
No growth = gram +; Non- fermenters - white/colorless
HE agar use
Select for gram negative bacteria; differential for lactose fermenters
HE agar media
Hektoen enteric agar
HE agar + result
growth = gram -; fermenters - salmon
HE agar - result
No growth = gram +; Non- fermenters - blue to blue- green
PEA agar use
Select for gram positive bacteria
PEA Agar Media
Phenylethyl Alcohol Agar
PEA agar + result
Growth = gram +
PEA agar - result
No growth = gram -
MS agar use
Select for
Staphylococcus;
differential for mannitol fermenters
MS AGAR media/ reagents
Mannitol salt agar, phenol red
MS agar + result
Growth = gram +; fermenter - yellow
MS agar - result
No growth = gram -; non- fermenter - red
Caseinase Production use
Detect if bacteria can break down casein (C. violaceum)
Caseinase production media
Milk agar
Caseinase production + result
Clear zone around bacterial growth
Caseinase production - result
No clear zone
Gelatinase production use
Detect if bacteria can break down gelatin (S. aureus, L. monocytogenes
Gelatinase production media
Nutrient gelatin
Gelatinase production + result
failure to solidify at cold temp
Gelatinase production - result
Solid at cold temp
Lipase Production use
detect if bacteria can break down tributyrin
Lipase Production media
Tributyrin agar
Lipase Production + result
Clear zone around bacterial growth
Lipase Production - result
No clear zone
Amylase Production use
Detect if bacteria can break down carbs (C. diphtheriae, C. perfringes, B. anthracis)
Amylase Production media/reagent
Starch agar, Iodine
Amylase Production + result
Clear zone around bacterial growth when iodine is added
Amylase Production - result
Purple color after iodine added
Carb Fermentation use
Detect if bacteria can ferment different carbs
carb fermentation media
Durham tubes, Fermentation broth + carb (glucose, lactose, sucrose), phenol red
Carb Fermentation + result
Yellow color
Carb Fermentation - result
Red color
Nitrate Test Use
Identify the way nitrate is reduced in certain bacteria
Nitrate Test media/ reagent
Nitrate reagent A&B, Zinc
Nitrate Test + result
Red after adding reagents A &B; if added zinc- colorless
Nitrate Test - result
Remained red after adding zinc
Oxidase test use
Detect cytochrome c oxidase activity (All Enterobacteriacease are negative)
oxidase test media
TMPD
Oxidase Test + result
Purple
Oxidase Test - result
Colorless
Catalase Test use
Test for Catalase Production
Catalase test media
hydrogen peroxide
Catalase test
+ result
Bubbling
Catalase Test - result
No bubbling
Indole test use
Test for Ability to Break down Tryptophan and yield indole
Indole Test media/ reagent
Tryptone broth, Kovac's reagent
Indole Test + result
Red color on surface of broth
Indole Test - result
Yellow
Methyl Red Test use
Ability to ferment glucose and form acids
Methyl Red Test media
MR-VP broth, Methyl Red
Methyl Red Test + result
Red
Methyl Red Test - result
Yellow
Voges- Proskauuer Test Use
Detects presence of acetoin
Voges- Proskauuer Test media
MR-VP broth, Barritt's reagent
Voges- Proskauuer Test + result
Deep pink
Voges- Proskauuer Test - result
no color change
Citrate Test use
Detect ability to utilize citrate acid as sole source of carbon
Citrate test media/reagent
Simmon's citrate agar, bromthymol blue
Citrate Test + result
medium turns from green to deep blue
Citrate Test - result
No color change - remain dark green
Phenylalanine Deamination use
test for phenylalanine deaminase - most bacter that produce this are pathogenic (ie Proteus, Morganella
Phenylalanine Deamination media/reagent
Phenylalanine slants, 10% ferric chloride
Phenylalanine Deamination + result
Green color on surface of medium
Phenylalanine Deamination - result
No color change
Lysine/Ornithine Decarboxylation use
Test for lysine and ornithine decarboxylase (one way to overcome IMViC tests limit.- p.63)
Lysine/Ornithine Decarboxylation media
Lysine & Ornithine broth, mineral oil, Bromcresol purple
Lysine/Ornithine Decarboxylation + result
Purple
Lysine/Ornithine Decarboxylation - result
No color change - yellow
Hydrogen Sulfide Test use
Detect hydrogen sulfide gas production
Hydrogen Sulfide Test media
Peptone iron agar deep containing ferrous sulfate
Hydrogen Sulfide Test + result
Black precipitate
Hydrogen Sulfide Test- result
No change
Urease Test use
Detects urease production; useful in distinguishing Proteus
Urease Test media/reagent
Urea broth, phenol red
Urease Test + result
Fuchsia/magneta
Urease Test - result
Yellow/orange
The type of microscope most often used in the laboratory is referred to as the _______ microscope?
brightfield
Functions of microscope
magnification and resolution
Parfocal
Property of microscope which allows objectives to be changed without having to refocus
Parcenter
when image is centered in field of view it will remain there when objective is changed
What is simple staining?
when one stain is used to examine shape and arrangement of bacteria
Direct staining uses
Basic stains
Negative staining uses
Acidic stains
heat-fixing is only used for _____________
basic stains (direct staining)
E.coli gram stain
pink colored rods, gram negative
M. luteus gram stain
purple colored, gram postive
crystal violet gram stain
primary stain
iodine in gram stain
mordant
95% alcohol gram stain
decolorizer (critical step)
Safranin in gram staining
counterstain
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