64 terms

MICRO FINAL LAB

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sugar fermentation has what control
Proteus Valgaris
Which control will be positive for Starch Hydrolysis and casein hydrolysis
bacillus subtillus
Which control will be positive for sugar fermentation, mixed fermentation, nitrate production, and tryptophan hydrolysis
E. coli
Which control will be positive for butandiol fermentation and citratase
Enterobacter aerogen
Which control will be positive for catalase and lipid hydrolysis
S. aureus
Mixed acid fermentation has what control
E. coli
Butandiol fermenation has what control
Enterobacter aerogen
Catalase has what control
Staphloccocus aureus
Oxidase Production has what control
Pseudomonas Aerginosa
Nitrate has what control
E. coli
Starch has what control
Bacillus Subtillus
Casin Hydrolysis has what control
bacillus subtillus
Lipid Hydrolysis has what control
staphylococcus
aureus
Tryptophan has what hydrolysis
e. coli
Urea hydrolysis has what control
Proteus Valgarius
H2S production has what control
Proteus vulgaris
Citrate Utilization has what control
enterobacter aerogenes
Phenylalanine Deamination has what control
proteus valgaris
Medium: phenol red broth
Appearance: yellow
Control: e. coli
sugar fermentation
Medium: MR
Enzyme: fir mite hydrolysis
Control: E. coli
Appearance: red/pink
Reagent: methyl red
Alt name: methyl red
mixed acid fermentation
Medium: VP
Control: enterobacter aerogen
Appearance: red
Reagent: Barrits reagent
Alt name: Vogues Prokauer test
Butandiol test
Medium: any
Enzyme: catalase
Control: Staphylococcus aureus
Appearance: bubbles on plate
Reagent: hydrogen peroxide
catalase
Medium: any
Enzyme: oxidase
Control: pseudomonas aerginosa
Appearance: purple
Reagent: oxidase reagent
Oxidase production
Idole: +
Methyl Red: +
Vogues Poskaur: -
Citrate: -
E.coli
What did typhoid mary have?
salmonella typhi
Idole: -
Methyl Red: -
Vogues Proskaur: +
Citrate: +
E. aerogenes
Medium: starch agar
Enzyme: amylose
Control: bacillus subtillus
Appearance: clear areas adjacent to starch growth
Reagent: Gram's Iodine
Starch Hydrolysis
Medium: skim milk agar
Enzyme: casinase
Control: Bacillus Subtillus
Appearance: yellow with clearing
casein hydrolysis
Medium: spirit blue agar
Enzyme: lipase
Control: Staphylococcus Aureus
Appearance: streak of blue precipitate
Lipid Hydrolysis
Medium: typtase broth
Enzyme: tryptophase
Control: E. coli
Appearance: red ring at the top
Enzyme: Kovacs reagent
Tryptophan Hydrolysis
Medium: SIM
Enzyme: cysteine disulferase
Control: Proteus Valgarius
Appearance: Blue precipitate
H2S production
Medium: Simmon's citrate slant
Enzyme: citatase
Control: Enterobacter aerogenes
Appearance: Blue
Citrate utilization
Medium: Phylalanine agar
Enzyme: Phylalanase
Control: Proteus Valgarius
Appearance: Deep green
Reagent: ferric chloride
Phenylalanine Deamination
Staphylococcaceae
family for gram positive staph
dz for staph
abscesses, boils, carbuncles, wound infections, toxic shock syndrome
Medically significant types of staph
S. aureus (most pathogenic)
S. epidermidis
S. saprophyticus
Family name for: streplococcaceae and Enterococcaceae
streptococcus
strep dz:
pneumonia, meningitis, pharyngitis, endocarditis, glomerulonephritis
Gram possitive, catalase neg
strep
Medically significants species
S. pyogenes --> rheumatic fever
S. agalactiae
S. pneumoniae
alpha hemolysis strep
partial clearing green color
beta hemolysis strep
complete clearing
gamma hemolytic strep
no clearing
Bacitracin +
clearing
S. pyogenes
Lancefield Group A
SXT +
any size zone of inhibition
Lancefield Group C
CAMP +
arrow head shape
S. agalactiae
Lancefield Group B
Bile esculin hydrolysis +
Blackening of slant
Lancefield Group D
Na tolerance +
turbidity in 24 hrs
group D
enterococci
Optochin susceptibility test +
zone of inhibition
S. pneumoniae
no lancefield antigen
neutrophils
most common granulocyte
50-70%
1.5-2 x RBC
high in infection
Eosinaphils
granulocyte
1-5%
2X size of RBC
cherry stem necleus
very red
high in parasitic infection
Basophils
granulocytes
<1%
1-3X size RBC
a lot of purple granulocytes
high in allergic reactions
Lymphocytes
agranulocytes
20-30%
same size as RBC
have a large nucleus
different than basophils by blue sac
high in viruses
Monocytes
2-6%
>3x RBC
crescent nucleus
also blue in sac
high in virus
infectious dz
dz caused by a microorganism that enters the body and replicates in tissues at the expense of the host organism
Epideomiology
the study of who, what, when, where, why, and how a dz is spread
epidemic
increase in number of reported dz in a given period of time within specific area
pandemic
an epidemic that is geographically widespread to other countries
If i have a beta streptococcus swab what next?
CAMP test (swab on a plate)
bacitracin and SXT stamped (antibiotic)
Use S. aureus
What do results of a BAP for beta streptococcus mean? CAMP -
Bacitracin s (any size zone)
SXT R
Group A S. pyogenes
What do results of a BAP for beta streptococcus mean? CAMP +
Bacitracin R
SXT R
group B S. agalactiae
What next? You identified your pt had alpha hemolysis streptococcus
one plate with swab and optochin
bile esculin slant
Alpha hemolysis + BAP
zone of optochin >14 mm
bile esculin slant -- brown -
Alpha hemolysis -BAP
optochin R
Viridans Streptococcus
bile esculin slant -- brown -