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Terms in this set (16)
If you see two different colony colors or sizes can you do further bacterial pure isolations?
because obviously there is more than one colony (more than one organism) on the plate and isolation has not been achieved since two different colors/sizes of colony
Two ways that objects or reagents can be sterilized?
autoclaving or radiation
Live organisms cannot be made sterile. True or false?
If E coli grew more on a plate in an aerobic conditions but less (but still grew) in anaerobic conditions then it is a __________________________ ____________________.
Why would a facultative anaerobe show less colonies on an anaerobic plate than an aerobic plate?
because it can grow in both conditions but since it grows "better" in the presence of oxygen it grew in larger amounts (more colonies) since it could yield more ATP with oxygen as its electron acceptor
We rolled our agar deeps between our hands when inoculating them to isolate which if these:
all of these
agar is kept liquid at
The term "sterile" is absolute. An item is either sterile or not sterile. True or false?
Facultative anaerobes grow how in an agar deep tube?
all over but most of the bacteria is growing on the top
Bacterial growth is observed on an agar slant, what can you assume about this bacteria?
Ut is facultative OR strictly aerobic
A microaerophilic organism
grows best in reduced oxygen but normal atmospheric conditions
What is likely the identification of a lactose fermenting organsim on the EMB plate?
(it will appear as marroon colonies)
how is EMb selective?
inhibits gram positive bacteria
how is emb differential?
lactose fermenters (maroon colonies) versus non lactose fermenters
One advantage of using plate count method over other methods?
it only counts bacteria that are viable (which are most important)
Trypticase soy agar is an example of ______ media
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