Biochem Exam 2 multiple choice
Terms in this set (13)
What is true about the relationship between protein misfolding and disease?
Amylodoses are a collection of diseases stemming from insoluble amyloid fiber deposition
The plot shown at the right shows fraction of ligand bound vs ligand concentration. What is the appropriate Kd and Ka, respectively, for the cooperatively-binding ligand?
x = kd (turning point)
y = 1/kd
Circle all of the ligands for hemoglobin that help successive O2 molecules to bind.
O2 and CO
Which is not something that aids in increasing the speed of an enzyme-catalyzed reaction
energy of productts
Choose the correct designation for the glycosidic linkage
(B = up) (alpha = down)
Choose the picture that shows the sugar code as part of a cellular recognition process
Which of the following are necessary to sequence DNA in the modern way
Vitamin C is NOT directly involved in the enzymatic chemical reaction that produces formed collagen
In an average healthy individual, 1% of less of his/her hemoglobin is complexed with CO. Which factors promote the complexation of hemoglobin with CO in people (these people may have more than 1% of the hemoglobin bound to CO)
working on faulty generators/motors
When 454 mg sample was reacted with the Sanger reagent, fluro dinitrobenzene (FDNP), 16.0 mg of DNP-Met were recovered. How many polypeptide chains are in the overall molecule of the protein? The protein has a molar mass of 45,000 g/mol and DNP-Met has a molar mass of 314.24 g/mol. Express your answer in one sig fig.
Explain briefly why cooperative binding of oxygen is useful in the function of hemoglobin in terms of lungs vs peripheral tissue
Cooperative binding means each successive ligand binds more easily. This suits the function of Hb because it can more readily take up O2 in the lungs where O2 is abundant and then release it more readily in the tissues where O2 is less abundant. A changing affinity allows for Hb to adapt to the concentration of O2 available.
Hemoglobin has a ___________ affinity of oxygen at lower pH and the ___________ of hemoglobin is favored. The effect of pH on oxygen binding in hemoglobin is known as the ____________.
Calculate the value of Km if 3.0nM of enzyme Q is reacted with 5uM substrate. The reaction has a Kcat of 500 sec-1 and an initial velocity of 250 nM/sec